Differential and continuous separation process with controlled paramenters for solids and liquids

ABSTRACT

By regulating separation system&#39;s relative parameters, this broad and generalized separation process is disclosed to distinguish the fundament between this invention and chromatography for superior cost-effectiveness. A different mass transfer contacting method and differential set-up between two phases are applied on the disclosed apparatus to achieve purposed efficiency. This continuous separation process can be furnished as the integration of multiple partial fluidized beds or cells; operated under designated pressure level to instantaneous and simultaneous proceeding of determined mass-transfer phenomena. All zones within the process are simultaneously proceeded to isolate one product or multiple products from feed mixtures with other proceeding zones of feeding, impurity stripping, regeneration and washing. The differential mass transfer contact method is disclosed for efficient consumption of both solid phase and mobile phase. The unique recycling technique is disclosed to efficiently reuse the mobile phase and concentrate all isolated components within process. The liquid delivery module and holding tanks are illustrated for increasing operation efficiency and flexibility.

This is a division of application Ser. No. 09/274,709 filed on Mar. 23,1999 issued as U.S. Pat. No. 6,280,623 on Aug. 28, 2001.

BACKGROUND OF THE INVENTION

1. Technical Field

This application is an extension of application Ser. No. 25,066 filedMar. 12, 1987, U.S. Pat. No. 4,758,349. To be more specific, thisinvention is broadly related to an apparatus and methods to isolate adesired component or multiple components simultaneously, from a solutioncontaining a plurality of mixture components. The apparatus is advancedby its unique construction to convey the novel separation method toleave the native engineering drawbacks of current chromatographicprocess become irrelevant, such as back mixing and diffusion, axialdispersion, and column end effects. By applying the differential set-upbetween solid and liquid phase, new mass transfer contacting method, andoperation protocols, this apparatus dramatically increases mass transferefficiency and effectively utilizes packing materials in comparison withchromatographic process under same feeding capacity requirement. Theprocess further controls the system separation parameters to avoiddeterioration of separation efficiency due native engineering drawbacksof chromatographic process.

2. Description of Prior Art

The review of prior literature and current survey of commercialchromatography involves with chemically adsorption and de-adsorptioninteraction of so called mass transfer phenomena between the solid andcomponent mixtures in liquid phase. It indicates the packed or fixed bedbatch type columns are the dominant device being widely used forseparation. Ever since 1967, the liquid chromatography was first emergedby Huber and Hulsman for faster and easier analytical speed over gaschromatography. Until nowadays that simulated moving bed processemploying the embodiments as described in U.S. Pat. Nos. 3,761,533 and3,201,491 become well known and adopted for such purposes. In thoseknown processes, a chromatographic column is divided into severalsections by distributors that allow fluid to flow into or out of eachsection. Those sections are interconnected in order and continuouslycirculating fluid stream flowing through all sections by circulating theeffluent fluid from an outlet of the last section to an inlet of thefirst section. At a setting time intervals, all the points ofintroducing and withdrawing the inlet and outlet streams are shiftedsimultaneously as same direction of fluid flow; this gives the packingmaterials a simulated flow in opposite direction of fluid flow.Alternatively, the multiple columns continuous solids and liquidscontacting device, as taught by the U.S. Pat. No. 4,522,726 andcontinuation-in-part-of U.S. Pat. No. 4,522,726 which is U.S. Pat. No.4,808,317. This device is described by its structure to provide fixedinlet and outlet nipples for introducing and removing fluid streams. Theinlet and outlet nipples are interconnected by a rotating columns whichare divided into three or four sections, similar to above mentionedsimulated moving bed process. By the nature of ratable construction ofits member and plural sections, a discrete fluid streams maysimultaneously betreated.

All of above mentioned processes comprise certain differences. However,they are all fallen into same categories that apply a stationary packingmaterial within the circular column with an attempt somewhat inmanipulating the column configurations and optimization in fluiddistribution. The separation is achieved through sequential stages asfeed adsorption, selectively desorption to elute impurities and productvia specific elution streams, adsorbent regeneration and washing. Asthumb of chromatographic operation, the liquid stream is being pushedfrom one end of column and the existing fluid to emerge from the otherend of column. When the fresh feed is delivered, the adsorption ofsolute components onto the adsorbent occurs. The adsorbent is beingconsumed and saturated with solutes slowly from one end of the column asfeed stream flow direction. At any given instance, the mass transfer isproceeded in a very small trace of adsorbent wherein the mixed zone oftwo consecutive liquids travels through the bed. The rest of the packingmaterials are idle for either as saturated adsorbent or waiting to besaturated. The adsorbent is inefficiently being consumed mainly becausethe idle of most part of packing materials. The same situation isrepeated for elution and regeneration stage. In view of unemployedpacking materials, the required amount of mobile phase is proportionalincreased with processing time in which the cycle time engaged for, eachconsecutive stage are accumulated for stages of feeding, multipleimpurity stripping and regeneration. For instance of eluting aparticular solute component, the specific fresh eluent is required topush from one end of the column and travel through the entire bed toemerge from the other end of column. This shall be compelled to createdilution of separated fractions due engineering drawbacks, of whichoften involves with the combined effects of axial dispersion, backmixing, and end effects for fluid in and out of column. Those combinedfluid dynamic phenomena have great impacts on product purity and processefficiency such that the feed loading limit is crucial and the separatedpeaks may often mixed up again with the neighboring peaks. Theproduction rate has to be compromised with purity. Thus, the separationefficiency is low. The mass transfer mechanism justifies thechromatography has natural deficiencies on loading limitation anddeteriorating separation, particularly in large industrial separationprocess. The typical batch chromatographic operation is a tediousprocess resulting in considerable downtime and often requiringcomplicated protocols and expensive support systems. The scale up of atypical column process for large-scale purification usually battles withnot only the loading limitation for acceptable quality but also requireshigh-pressure operation and high-energy consumption. Furthermore, thescale up usually is linear procedures from pilot scale through whichinvolves all concerns of not only cost of construction but higherprobability of failure.

It is readily understood that the breakthrough of efficient consumptionof packing materials not only shall provide a gifted separation processto triumph the native engineering deficiencies of traditionalchromatography. It also markedly improves the efficiency of existingseparation processes in aspects for low operation cost, low equipmentinvestment, and flexible separation protocols.

SUMMARY OF THE INVENTION

A principle object of the present invention is to provide methods and acorresponding apparatus for continuous separation of combining stages offeeding, impurity stripping, adsorbent regeneration, and washing. Theintegration of all stages represents a complete separation cycle. Itmeans one stream or multiple streams of products can be simultaneouslyisolated with multi-impurities stripping through continuous execution ofdisclosed apparatus. Each separated fraction is simultaneously recoveredwithin a specific zone of the apparatus. Each zone is corresponding toreflect one of the aforementioned stages. Each zone contains one or morethan one cell. The cell means a tall chamber installed with adsorbentand acts like a partially fluidized bed or mixed reactor, which receivesthe fluid from top inlet to instantaneously and partially up lift theadsorbent retained in such chamber. At least one cell is composed as azone representing a predetermined mass transfer task implemented fromthe predetermined elution profile of a target separation system. Allcells are mounted on a circular plate and can be rotated and stopped fora predetermined rotation angle through a rotating and positioningmechanism. A stationary lower compartment is partitioned into severalzone compartments which is located between holding tanks and rotarygroup of cells for collecting the drained fluids via driving forces ofvacuum and pressured gas or air. Holding tanks, located under each zonecompartment, are provided as reservoirs for temporary reserving fluidscollected from corresponding zones and redistributing fluids for variousapplications. Vapor recovery units are provided between the mainassembly of the apparatus and the corresponding vacuum pump forrecovering the fume and reuse the condensed liquid.

Unlike chromatographic operation, the resident resin is stationary andconstantly maintained in wet status with surrounding liquid and thefluid is progressively pushed from one end of column and exited theexisting fluid out from other end. The elution starts after at least onebed volume due involvement of column dead volume and resin void volume.The main object of the present invention is to create a new masstransfer contact method. It comprises maintaining the resin constantlyin semi-dry status, meaning the resin may have wet surface and no liquidexists between resin particles. It is further object by splashing apredetermined volume of liquid from top of cell onto such semi-dry resinfor generating a partially fluidization between suspended resin in theliquid phase to instantaneously promoting a homogenous mass transfercontact. It is further object to periodically input the pressure gas orair from top of each cell, in between the ceased liquid delivery, toaffiliate in part with continuous vacuum to immediate settle and drainthe liquid through the bed. This illustrates the new mass transfercontact method. The method has created a zero dead volume process,meaning the elution starts from the very beginning of bed. Nevertheless,the process has similar elution profile to that of chromatographicoperation.

It is a further object of this invention to provide a differentialset-up to convert both adsorbent and mobile phase for complete usage ofadsorbent and thus obtaining the maximum mass-transfer efficiencybetween two phases. The conventional chromatographic mass transfer pathis parallel to the mobile phase's flow direction and the solid phase isstationary. Inversely, this disclosure continuously and homogeneouslyexposes the solid phase actually moving in horizontal direction toperpendicularly in contact with mobile phase. It is achieved throughswift mass-transfer contact by the implementation of said new masstransfer method and differential set-up between two phases. It istherefore the ultimate object of this invention to dramatic reduction ofresin inventory.

It is a further object of this invention to define a new mobile phaseinput mode to distinguish that from chromatography. The input S-I modeis defined as that predetermined volumes of same mobile phase conditionare simultaneously delivered within the minimal time interval into eachcell located in same zone. The input I-I mode is defined as thatpredetermined volumes of discrete increments of mobile phase conditionsare simultaneously delivered into each cell located in same zone withinthe minimal time interval. The differential increments of mobile phaseconditions are predetermined between two designate levels that aregrouped with each corresponding cell in such zone. The input volume ofeach discrete mobile phase condition is predetermined and tied in withdesignated cell in such zone as well.

It is further object of the invention to provide a preferred operationprotocol to manipulate the preferred apparatus in conjunction withprevious objects to implement the differential set-up and new masstransfer method. The operation protocol includes three preferredoperation stages, which are start-upstage, steady state stage, andtermination stage.

It is a further object of the invention to provide single-stage recyclefor simultaneous isolation and enhancing the concentration level ofseparated fractions and economic consumption of mobile phase. Thecollected fluids are temporarily stored in holding tanks located undereach corresponding zone compartment of preferred apparatus to recycleback to same zone and/or other neighboring zones to achievesuch-purposes.

It is further object of the invention to provide recycle protocol inmultiple stages. The multiple-stage recycle is established with thepredetermined number of stages arranged in single series or multipleseries in parallel to further break down the required volume of feedinput. Each stage represents an execution of single apparatus. Through asteady flow of mobile phases travelling vertically within theestablishment, it simultaneously carries out the multiple componentsseparation along with the enhancement of concentration level ofseparated fractions and economic consumption of mobile phases. Yet, ittransforms the path of mass transfer interaction from parallel tovertical direction between the solid phase and mobile-phase's flow routewhereas the solid phase is partially fluidized to perpendicularlycontact with mobile phase. This is fundamental from the characteristicelution profile of a particular separation system investigated under thecriterion of said new mass transfer method and differential set-up. Theimplementation of such elution cycle covering from stages of loading towashing are simultaneously proceeded by the process within every spentof predetermined minimal time interval.

It is further object of the invention to extend to other chemical unitoperation constitutes resembling mass transfer behavior through combinedapplication of differential set up, new mass transfer method, operationprotocols, and preferred apparatus. The applications are such ascatalytic reaction in pack tower and fluidized solid and liquidreaction.

It is further object of the invention to provide a preferred fluiddelivered and recycle system to provide easy and adequate processcleaning and sanitation application.

The disclosed invention is achieved by a method for separating onecomponent, or at least one component, simultaneously from plurality oftarget mixtures. The complete methods are composed of the integration ofaforementioned objects and carried out by the apparatus.

BRIEF DESCRIPTION OF THE DRAWINGS

The above and other objects, distinct features and advantageous of thepresent disclosure can be more readily illustrated from the followingdescription, taken with drawings in which:

FIG. 1 is the schematic perspective view of preferred embodimentmechanical set up,

FIG. 2a is the cross-sectional profile of preferred process set up ofFIG. 1; and FIG. 2b is the blown-up view of preferred two alternativesof rotation and seal mechanism related to FIG. 1 and FIG. 2a;

FIG. 3 is the general perspective view of preferred cell constructionfor implementation of instantaneously partially fluidized effect betweenresident resin and upcoming fluid; particularly for single type resin;and

FIG. 4 is the general perspective view of preferred cell constructionfor single type of resin in multiple layers, or multiple kinds of resinin multiple layers;

FIG. 5 is the schematic illustration of three input modes inchromatographic operation and their outcome of loading and elutionstage;

FIG. 6a is the method to implement for continuous and simultaneousisolation of single product A and the two groups of impurities B1 andB2, and FIG. 6b is a generalized scheme to carry out the differentialset up;

FIG. 7 is the method to implement for continuous and simultaneousisolation of multiple products in conjunction with a generalized schemeto carry out the differential set up; and

FIG. 8 is an illustrated example, the generalized scheme demonstratedfor the isolation of two products A1 and A2 and impurities groups of B1,B2, and B3;

FIG. 9 is a schematic flow diagram representing the single stage recycleprotocol; and

FIG. 10 is a schematic flow diagram representing multiple stages recycleprocedures;

FIG. 11 shows three elution profiles of chromatographic cyclicadsorption and desorption of Hemoglobin to demonstrate the iso-pointbelief as guideline for employing the new mass transfer method,conversely;

FIG. 12 is an elution profile of high-fructose corn syrup separated bychromatography and

FIG. 13 is an elution profile of high-fructose corn syrup separated bynew mass transfer method described in FIG. 3;

FIG. 14 through FIG. 17 are composed by four consecutive steps ofrecycle procedures to illustrate the advantages of employment of newmass transfer method and differential set-up; and

FIG. 18 is the schematic flow diagram for demonstrating the recycleprotocol through which the comparison of resin stock, eluent waterconsumption, and product recovery been evaluated between thechromatography and present disclosure.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

This invention includes an apparatus and the integration of new masstransfer method in conjunction with differential set up between twophases and operation protocols to implement the prefixed separationsystem's parameters onto the apparatus. Both the apparatus and methodsare interrelated as hybrid embodiments and illustrated in fourdescriptive embodiments with aforementioned drawings. The firstconstituent starts from the preferred apparatus described in FIGS. 1, 2,3, and 4. The second constituent involves the various mass transfernovelties and methods covered from FIG. 5 to FIG. 8. Then, the FIGS. 1and 2 are studied again for the implementation of generalized operationprotocol. Moreover, FIGS. 9 and 10 are illustrated to introduce thesingle stage and multiple stages recycle protocols. Finally,experimental data are demonstrated via FIG. 11 through FIG. 18 fornumerous advantageous of this disclosure over current column process. Itis clear that all drawings and examples are mainly for illustration andpossible extent of alternation or configurations of mechanicalstructures may be explored. Yet, fundamental concept of this inventionshould set above such possible modification and be within the scope ofthis invention, mainly because this invention is the hybrid of anapparatus with broad and generalized mass transfer methods.

FIG. 1 shows the perspective view of the preferred apparatus 10 broadlyindicated as the sophisticated separation process. It contains aplurality of cylindrical cells 11, holding a predetermined quantity ofpacking materials 12, mounted equally in one or multiple layers alongthe circumference of a circular plate 13. Only two layers of cells aredrawn for simplicity. The layer means circumference of different radiusfrom the center of circular plate 13. The micro bead shaped packingmaterials are classified in various categories and may be anycommercially available adsorbent used in chromatography that canchemically and selectively interact with the dissolved components inmobile phase to promote successive separation. The whole group of evenlyspaced cells 11 is subdivided into several zones to conduct specificmass transfer task and classified as feeding zone 14 impurity strippingzone 15, product recovery zone 16, regeneration zone 17, and washingzone 18. Certain zone may be further divided, depending on how manyextra components are to be simultaneously recovered. An endless circulartrough like curved segmental chamber 19 located beneath eachcorresponding zone is partitioned to cover about same range of cells aszone compartment 20, 21, 22, 23, 24 to collect the fluid drained fromsuch zone. A matching holding tank located under each corresponding zonecompartment as tanks 25 (not shown), 26, 27, 28, and 29 (not shown) fortemporary storage and redistributing the collected fluid from such zone.Each zone compartment is equipped with a vapor or fume recovery unit 30(not shown), 31, 32, 33, and 34 (not shown) as means for vapor recovery.The recovered liquid is temporary stored in tank located beneath eachcorresponding vapor recovery unit as tank 34 (not shown), 35, 36, 37,and 38 (not shown) for future treatment such as filtration and recycledor discarded. Vacuum pumps 39 (not shown), 40, 41, 42, and 43 (notshown) are installed corresponding to each vapor recovery unit asdriving force for liquid draining. Solenoid valves 44 (not shown), 45,46, 47, and 48 (not shown) are located in the connection above eachcorresponding holding tank. Valves are usually set at closed position tomaintain vacuum for liquid draining and switch to open position tocoordinate the liquid flow into such tank during the rotation of plate13. The fluid delivery module composed five manifolds 49, 50, 51, 52, 53installed in accordance above zones 14 to 18. Fluid is electronicallydelivered through a solenoid valve 54 located right above each cell 11.Another solenoid valve 55 located next to each valve 54 is for input ofpressure air or gas to affiliate vacuum for fluid draining. An endlessflattop reversed “U” shape annular channel 56 is installed near vicinityof circumference of plate 13 to cover all cells and provided assupporting platform for mounting all valves 54 and 55. The interior wallof compartment 56 may be modified with either heating or cooling jacketfor temperature control, installed with insulation for temperaturesensitive operation, or other extended features and configurations forspecial applications.

In FIG. 2a indicates the cross-sectional profile of FIG. 1 forillustration of first option of rotation and seal mechanism. Valves 54and 55 are mounted on top of each cell 11 and secured at predeterminedpositions of flattop compartment 56. The compartment 56 has two rimsmounted on flat top surface of concentric railings, installed along thecentral axis. The inner rim 57 is secured on inner-upper railing 58 andouter rim 59 is secured on outer-upper railing 60. A matchinginner-lower railing 61 and matching outer-lower railing 62, both haveflat bottom smooth surface to be tightly fastened with top surface ofplate 13. The curved bottom segmental chamber 19 has similar upright “U”shape annular channel as compartment 56, having two rims attached to twopairs of duplicated concentric railing installed along the central axis.The inner rim 63 is fastened with inner-lower railing 64 and outer rim65 is fastened with outer-lower railing 66. A matching inner-upperrailing 67 and a matching railing 68, both are tightly fastened on lowersurface of plate 13.

FIG. 2b, the top view of FIG. 1, further illustrates two preferredrotation and seal mechanisms by an exploded profile; and manifolds,solenoid valves, and cells are not shown for readily demonstration.Briefly repeating earlier illustration of first option concerning thisfigure; rims 57, 59, 63, and 65 represent the part section ofcompartment 56 and 19. Those rims are tightly mounted on railing 58, 60,64, and 66 respectively as shown on left part of FIG. 2b by a blown upview. The matching railings 61 and 62 are secured on top-surface, and 67and 68 are mounted beneath the bottom-surface of plate 13 of which isevenly and horizontally secured in between four pairs railing. For allrailings resemble in structure, just the pair of 60 and 62 isillustrated by exploded view for first option of rotation and sealmechanism. Railing 60 has a center down-face half-oval shaped groove 69to match the center up-face half-oval shaped groove 70 on railing 62.The combination of groove 69 and 70 generates a concentric oval-shapedtunnel 72 as demonstrated by railing pair of 58 and 61. Circular balls71 with its diameter slightly larger than height of 72 are laid withinsuch that a gap 73 is formed. There have circular grooves 74, 75, 76, 77to be shaped as concentric circular tunnel 78 and 79 to accommodateO-ring type seal 80 with its diameter just big enough to seal the gap73. It is clear that the configuration of railings, steel balls, andO-ring seals furnish a sufficient frictionless rotation mechanism forplate 13, providing minimal air leakage for compartment 56 to maintainpressure-air and compartment 19 to hold the vacuum.

A concise rotation and seal mechanism is further illustrated as secondoption shown on the right side of FIG. 2b and other resembling positionswill have same structure with variance in mounting locations. Bothcompartments 56 and 19 have a concentric flat flange extended asL-shaped rim, viewed from cross-sectional profile. The L-shaped rim 59is a part of compartment 56 and L-shaped rim 65 been a part ofcompartment 19. Both rims have identical flange width, which aresuitable to support plate 13 in between the compartment 56 and 19. Acolumn shaped bearing 81 with its diameter slightly larger than thecross-sectional depth of plate 13 is symmetrically inset within theplate at direction perpendicular to a imaginary tangent line 82. Suchthat the bearing 81 is evenly hanged over the surface of plate 13 by agap 83 to support the compartments 56 and 19. It is recommended toevenly install with at least a multiplicity of four bearings to havegood support and rotation capability. Two concentric face-uphalf-circular grooves 84 and 85 located on upper surface of plate 13 inwhich two O-ring seals 86 with its radius slightly smaller than gap 83are installed. Two concentric facedown half-circular grooves 87 and 88located beneath the surface of plate 13 in which two O-ring seals 86 areinstalled. The bearings 81 and circular shaped O-ring seals 86 are allinstalled between the range of flat flange extension of compartments 56and 19; therefore, the plate 13 can be free to rotate with minimal airleakage.

As referring back to the center part of FIG. 2a, a rotation andpositioning mechanism is provided through a center mounted positionmotor 89 equipped with a speed reducer; usually gearbox 90 and a motorbrake 91. Motor 89 rotates the plate 13 via stretch bars 91 with allcells 11 mounted on plate 13 in clockwise or otherwise incounter-clockwise direction. The direction of rotation and the range ofone rotation step are preset and remain unchanged for one cell at a timeor more than one cell as a group. As shown from FIG. 2b, the range ofpredetermined rotation step is controlled by a light sensor 92 viaevenly drilled holes 93 near the circumference of plate 13. The distancebetween two adjacent holes 93 represents the range of a rotation step.The light sensors 92 mounted face to face on bracket 94 constantly emitslight beam, which is blocked by plate 13 when the plate 13 is moving.Initially, the motor 89 receives the signal from the controller toenergize and rotate the plate 13 forward. The plate 13 continuouslyrotates forward until the light sensors detect the light beam throughhole 93 in order to de-energize the motor and concurrently initiate themotor brake 91 to halt the motor and movement of plate 13. It shall beunderstood that there is no limitation of other alternatives of motioncontrol systems such as actuator or servomotor can be applied for suchpurpose.

The preferred construction of cell 11 shown in FIG. 3 is the essentialelement to implement the new mass transfer method and to distinguishthis disclosure from chromatography. The cell is a tall cylindricalcontainer retaining a predetermined quantity of resin by a porous filter95, which has porous mesh small enough to prevent resin from beingdrained while to maintain good permeable capability. The cell has anoutlet opening mounted by a seal gasket 96 on plate 13. The vacuum isconstantly engaged to maintain the resin in semi-dry status; meaning theresin may have wet surface but no liquid exists among resin grains. Asprevious illustration of rotation and positioning mechanism, all cells11 rotate one-rotation step and align to each cell's center position.The outlet end of solenoid valve 54 has a less than 180 degree baffle 97to conduct the predetermined volume of fluid to splash over like anumbrella shape. The splashed fluid hit the container wall, and slidedownward to penetrate and partially up-lift the grains of resinsuspended in liquid. This instantaneous partial mixing effect is forquick contact and dramatic reduction of required time for mass transferbetween two phases. Soon after the liquid deliver is completed, thepressure air or gas is released via solenoid valve 55 in part withexternal vacuum to immediately settle the bed. The drained fluidpenetrates through the remaining of the bed to complete mass transfercontact. Note that the fluid is drained means the solid phase returns toits initial semi-dry status throughout the whole bed. Thus, itdistinguishes the new mass transfer contact method from chromatography.The minimal time required for completing the new mass transfer methodincluding liquid delivery to fluidize bed, settled and drained thefluid, and advance one rotation step is defined as minimal timeinterval, Δt. This time interval is the mechanical limitation of theapparatus for liquid delivery and liquid draining and is the fundamentalparameter for converting chromatography into present invention.Preferably, the draining driving force has no limit of combinationsthrough other forms. The primary purpose is to affiliate the drainingspeed.

The predetermined quantity of one type of resin may be subdivided intomore than one thin layer to affiliate better liquid permeable capabilitythroughout the cell if pressure drop is too high. As shown in FIG. 4,each thin layer of resin is, supported by its own porous filter 98 ineach shallow bed 99. The total height of individual shallow bed 99comprises of a depth of resin and an opening space above. Each layer ofresin has a porous filter 98 to support such resin as the fluid outletof current layer and the predetermined open space as the fluid inlet ofnext layer. As shown by exploded view located on rightside of FIG. 4,each bed 99 is equally stacked up with such predetermined interval andsupported by a metal tube 100 stacked in between mounted washer 101. Theposition of washer 101 is located at bottom of each bed 99. A threadedmetal rod 102 with stud is pierced from top through all tubes 100 andwashers 101 and secured by a nut 103 to connect all the shallow beds 99in series. The outmost part of filter 98 has an extended and built inrubber annular shape gasket 104 with filter (only shown ascross-sectional cut), which is tied in between individual bed 99 toprevent air and liquid leakage. The stacked up structure of all bedswith an open space in between individual bed reduces the pressure dropto minimal.

The advanced cell construction is further expanded to accommodatemultiple kinds of resin via multiply-layer set up. The multiple-layerset up means a cell construction is as shown asFIG. 4. The multiple kindof packing materials means that more than one type of resin is packed indifferent zones of the cell construction. The zone means a groupednumber of layers 99 arranged as a zone interval within cell body toaccommodate one type of resin and additional zone for additional kind ofresin. All zones are constructed in series to vertically affiliate fastdraining via vacuum and pressure-air. Multiple kinds of resin can be anycombinations of anion exchanger, cation exchanger, reverse phase resin,normal phase resin, activated carbon, or other types of packing materialcan chemically interact with mobile phase to have adsorption andde-sorption capabilities. One fluid spraying baffle 97, similar to thatshown in FIG. 3, is installed at top of cell body to deliver fluid intofirst layer of cell. It allows a specific fluid with multiplecompositions of dissolved solutes to interact with each zone's resin tocomplete mass transfer interaction as the fluid drained throughout thecell body. The mass transfer interaction means either adsorption and/orde sorption phenomena shall be proceeded between liquid phase andmultiple kinds of resin within the predetermined minimal time interval,Δt. The minimal time interval is same as formerly defined.

As previous illustration of chromatographic operation, the feed solutionis pushed from one end of column to promote adsorption of solutecomponents onto the resin and simultaneously push out the existingmobile phase out from the other end of column. As shown in upper part ofFIG. 5, the dark region 103 represents the resin been saturated andprogressively increases as the feed input is prevailing in time domain.The unclouded area 104 represents the fresh resin that is not beensaturated with feed stream. As shown by loading stage in middle part ofFIG. 5, three input modes named as step input 105, pulse-input 106, andimpose input 107 generate same result as earlier illustration. Theshaded area 103 is named as mass transfer zone abbreviated as MTZ, and afresh zone 104 remains unsaturated. The zone 103 in general occupies5-15% of total bed volume and unused resin in zone 104 is soaked withliquid.

The feed input is stopped and then switched to various conditions ofelution liquid. The incoming fluid, in form of three input modes asshown in lower part of FIG. 5, will push the existing fluid out of thecolumn. The conjunction zone 108 of two mixing fluids as indicated astransient profile 109, representing the condition of mobile phase, isprogressively changing from feed toward elution liquid. Note that thistransient curve 109 starts from the very beginning of column in whichthe adsorbed solute components original in feed. Now, they have beenselectively departed from resin along with time prevailing and returnedto such elute liquid (mobile phase). The highest peak of elutedcomponent (only one is shown for illustration) points to its specificcorresponding mobile phase parameter namely as iso-point 110, at whichthe adsorbed solute starts to department from resin to return to mobilephase. In reality, it asserts an equilibrium status between solid andmobile phase that the adsorbed solute component will not depart from itsbounded site unless its surrounding mobile phase is favorable for suchphenomena to occur. It means every solute component has its own uniqueequilibrium status that only allow such solute component to be elutedfrom bonded status to mobile phase. It means a neutral equilibrium pointhas to be triggered for each component prior to its elution. This factrelates to all adsorbed components that each one may depart from resinonly if the surrounding mobile phase condition exceeds its neutral pointdespite flow dynamics near by.

From the above iso-point teaching, this invention utilizes identicalmobile and solid phases used in chromatography to proceed the separationthrough mixing or fluidized effect by controlling the mobile phase'scondition to contact with solid phase. It means through said new masstransfer method and impending illustration of differential set-upbetween two phases and operation protocol, implemented on saidapparatus, can obtain maximum separation efficiency within every spentof minimal time interval, Δt.

The optimal relationship between optimal resin quantity required for afixed volume of feed solution is determined by gradually adding andmixing the resin into such feed solution to promote solute componentsbeen adsorbed onto the resin. The optimal resin quantity means the exactamount of semi-dry condition resin is added to a fixed volume of feedsolution to promote at least one and/or all solute components beencompletely adsorbed. The current invention implements the optimalrelationship in proportion with the throughput requirement of feedsolution in terms of exact amount resin for complete saturation withsuch throughput. It means the resin installed in each cell of apparatusis equivalent to the mass transfer zone 103, formerly defined MTZ, whichis the zone of resin been saturated with the predetermined throughput inchromatography.

For establishing a generalized differential set-up for any targetsystem, it requires to produce an elution characteristic profile from asingle cell by applying the new mass transfer contact method asdescribed in FIG. 3 and/or FIG. 4. The FIG. 6a shows two modes ofschematic elution diagrams produced by this method. It is plotted by thesolute concentration on left-Y-axis and mobile phase parameter onright-Y axis, both vs. time on X-axis. Both modes represent ageneralized profile covering zones of feeding 14, impurity stripping 15,product recovery 16, regeneration 17, and washing 18; each zone iscorresponding to said apparatus in FIG. 1.

The first mode is called isocratic elution, input S-I. It is completedby five step-input 105, as defined in FIG. 5 with five runningconditions. By nature, the step input 105, is for interpretation ofrunning condition prevailed in time domain. As described in FIG. 3and/or FIG. 4, the small dose of liquid phase is delivered and sippedthroughout the cell before another dose is delivered. The type of liquidinput interpreted in time domain is actually as a form of impulse input107 as described in FIG. 5. The duration of liquid input in time domainis reduced to as small as possible, corresponding to the defined minimaltime interval Δt. Therefore, the definition of liquid input for thepresent invention is preferable described as step input in runningcondition but with impulse input in time domain, which is denoted asinput S-I. Mainly because the definition of step input forchromatography is not appropriate.

The second mode of input is called discrete isocratic elution, inputI-I. It is completed by inputting a group different mobile phases withminor increment of running parameter. The minor increment of runningparameter is defined as little change as possible and carried out withina minimal time interval Δt. It means an impulse input is defined forboth running condition and time, which is denoted as input I-I. Theduration of a minor increment of running parameter may be equivalent toone or multiple minimal time interval, which is de pending on targetseparation system. By proceeding the input I-I is purposely formaximizing the elution of current group and minimizing the elution ofsubsequent group to avoid the co-elution because the iso-points of twoadjacent groups are close to each other. The integration of both minorinput volume and its corresponding minimal time interval Δt for eitherinput S-I or input I-I, which is equivalent to total input volumeexecuted under total time duration. Therefore, the previous-illustration fore said apparatus makes the input S-I and input I-Ipossible to be distinguished from chromatography. In general, input I-Iis not required unless a better separation is demanded.

An illustrative elution profile for input S-I by sequentially startingfrom mobile phase #1 (feed condition) through #2, #3, to #4 createsthree peaks plotted in solute concentration (left-Y-axis) vs. timedomain and mobile phase parameter (right-Y-axis) vs. time domain. Thefirst step is feed loading, #1, onto fresh resin proceeded in loadingzone 14 to obtain the maximum adsorption efficiency; meaning to spendshortest possible tithe for maximum feed stock throughput. Allconditions of subsequent elution liquids are predetermined according theprevious teaching of iso-point belief. The mobile phase condition ispredetermined that #2 condition is preset prior to the iso-point ofdesired product A, thus, only impurities B1 can be eluted. This coversthe impurity-stripping step proceeded in impurity-stripping zone 15.Then, #3 mobile phase has its condition just elutes the desired productA and retains the impurities group B2 in solid phase. It covers theproduct-recovery step proceeded in product-recovery zone 16. The #4condition is input to cover elution of impurities B2 as regenerationstep proceeded in regeneration zone 17. Finally, the washing step isproceeded in washing zone 18 by recycling of #1 mobile phase collectedfrom feed zone to prepare resin ready for loading again.

The input I-I is illustrated by starting from feeding step to obtain themaximum adsorption efficiency proceeded in feeding zone 14 with #1mobile phase. The impurity-stripping step is proceeded inimpurity-stripping zone 15 by input I-I, minor increment of mobile phaseparameter starting from #1 then ending by #2, to maximize the elution offirst impurity group B1 to avoid co-elution with product A. The productrecovery step is proceeded in product recovery zone 16 by input I-Istarting #2 then ending #3 mobile phase parameter for maximizing theelution of product A to avoid co-elution with 2^(nd). Impurity group B2.Generally, one increment of mobile phase parameter is sufficient foreach increment of minimal interval Δt. Once the product A is eluted, theregeneration step is proceeded in regeneration zone 17 by input S-I #4mobile phase to strip off 2^(nd). Impurity group B2 from solid phase.Finally, the washing step is proceeded in washing zone 18 by input S-Iwith #1 mobile phase recycled from zone 14 to prepare resin ready forzone 14 adsorption. Note that the resin in both modes of input S-I andinput I-I is always maintained in semi-dry status in accordance with thecriterion of new mass transfer method via vacuum and/or pressure air.Chromatography involves void volume that the front part of the peak willnot emerge from other end of column until all existing mobile phase incolumn is completely pushed out. The current elution profile consumesexact amount of resin and such profile starts from the beginning of bed,meaning that zone 104 does not exist in comparison with chromatography.Yet, the said cell in apparatus has no dead volume at all in fluiddelivering system. Note also from the previous illustration that zone104 in chromatography not only contributes nothing but also deterioratesthe separation.

For easier illustration of differential set-up between two phases ontosaid apparatus, the apparatus is simplified as an annular ring in thecenter of FIG. 6b. The schematic drawing indicates the preferredapparatus is divided into five parts corresponding to the feeding zone14, first impurity stripping zone 15, product recovery zone 16,regeneration zone 17, and washing zone 18, respectively. The variance insize of different zones shown in the annular drawing is intended toreflect the realistic time may actually be required for completing themass transfer contact for specific zone. Yet, it may be different fordifferent target system and may only require four zones simply becausethe binary systems. It means not necessary all target separation systemrequires five zones set-up, it relies on the nature of target system.This schematic drawing shall serve well for purpose of illustration.

The resin quantity 112 installed in each cell of apparatus is equivalentto mass transfer zone 103, formerly defined MTZ in chromatography. Itmeans the zone 103, representing by resin quantity 112, is the resinbeen saturated with the fixed feed volume 111 and is installed in eachcell of apparatus. From the elution profile of single cell evaluatedunder criterion of said new mass transfer described in FIG. 6a, suchresult can be implemented with said apparatus for differential set-up.Note the same amount of solid phase 112 determined in feed zone isinstalled for other cells in different tones.

The zones shown in FIG. 6b comprise loading zone 14, first impuritystripping zone 15, product recovery zone 16, regeneration zone 17, andwashing zone 18. The time spent for each corresponding zone, obtainedfrom FIG. 6a, is divided by same minimal time interval Δt in feed zoneto gain the number of optimal steps for such zone. The predeterminedminimal interval Δt is interpreted, as primarily for the time requiredfor such partial volume of liquids to simultaneously sip through eachcell via vacuum and/or pressure air. The number of optimal steps isequivalent to number of cells or groups of cells as one advance stepinstalled in each corresponding zone. The type can be input S-I forisocratic elution or input I-I for discrete isocratic elution inaccordance with earlier illustration. The input S-I only differentiatesthe volume of same mobile phase condition, meaning the partial volumedelivered within every spent of minimal time interval for each cell isobtained from total liquid volume divided by number of optimal steps.The input I-I differentiates both the input volume and mobile phasecondition in accordance with each minimal time interval spent. Eachpartial input volume is a discrete increment of mobile phase conditionfallen in between two adjacent conditions with each corresponding numberof step. In reality of liquid delivering module of input I-I fox saidapparatus, such preset intermediate mobile phase's condition isseparately delivered in such partial volume for the corresponding cellthat stops underneath to receive such liquid dose.

The time spent for feed loading, under criterion of maximum adsorptionefficiency, divided by minimal time interval to obtain the number ofsteps is the number of cells or group of cells as an advanced step inloading zone 14. After volume 111 divided with number of optimal steps,the pairs of partial feed volume 113 and amount of solid phase 112 isgrouped to constitute as a cell. The integration of all cells with timeallows one cell to accomplish loading within every spent of minimal timeinterval. As all cells simultaneously receive the volume 113 and alwayshas one cell or a group of cells as an advanced step moves out from zone14 to zone 15.

In first impurity stripping zone 15, the liquid volume 114 representsthe total volume for input S-I of condition #2 or the cumulative volumefor input I-I of liquids between #1 and #2. After volume 114 dividedwith number of optimal steps, the pair of partial volume 115 and resin112 is grouped to constitute as a cell. The integration of all cells inzone 15 accomplishes the elution of first impurity group within everytime interval Δt spent. As all cells simultaneously receive the volume115 and always has one cell or a group of cells as an advanced stepmoves out from zone 15 to zone 16.

In product recovery zone 16, the liquid volume 117 represents the totalvolume of mobile phase #3 for input S-I or cumulative volume for inputI-I of liquids between #2 and #3. After volume 117 divided by the numberof optimal steps, the pair of partial volume 118 and resin 112 isgrouped to constitute as a cell. The integration of all cells in zone 16accomplishes the elution of product A within every time interval Δtspent. As all cells simultaneously receive the volume 118 and always hasone cell or a group of cells as an advanced step moves out from zone 16to zone 17.

In regeneration zone 17, the liquid volume 119 represents the totalvolume of mobile phase #4 for input S-I. After volume 119 divided by thenumber of optimal steps, the pair of partial volume 120 and resin 112 isgrouped to constitute as a cell. The integration of all cells in zone 17accomplishes the elution of 2^(nd) impurity group within every timeinterval Δt spent. As all cells simultaneously receive the volume 120and always has one cell or a group of cells as an advanced step movesout from zone 17 to zone 18.

In washing zone 18, the type of input is input S-I and liquid volume 121of mobile phase #1 can be recycled from fluid collected in zone 14.After volume 121 divided by the number of optimal steps, the pair ofpartial volume 122 and resin 112 is grouped to constitute as a cell. Theintegration of all cells in zone 18 accomplishes the adjusting resinready for loading within every time interval Δt. Because the all cellssimultaneously receive the volume 122 and always has one cell or a groupof cells as an advanced step moves out from zone 18 to zone 14.

The differential set-up is further extended to simultaneous isolation ofplural streams of different product with concurrent and continuousexecution of feeding, multiple groups of impurity stripping,regeneration, and washing. Depending upon nature of the target system,it requires producing an elution characteristic profile from a singlecell by applying new mass transfer method described previously. A widevariance can be existed and end up with different separation protocol,however, the result of such profile is sufficient to designate variouszones for the apparatus. For easier illustration, FIG. 7 is a schematicdiagram to elucidate such extension for simultaneous two productsrecovery through seven zones set-up. It depends upon how the product andimpurities are defined to end up less than seven zones to recover morethan two products. It is therefore to specify that a desired product isisolated in between two impurities groups, considering that has threeimpurities groups and two products plus feeding and washing ends uptotal seven zones. The solute concentration is plotted on left-Y-axis asa function of cumulative time. Moreover, the mobile phase parameter isplotted on right-Y-axis as a function of cumulative time.

The illustrative elution profile for isocratic elution, input S-I mode,by starting from mobile phase #1 (feed condition) through #2, #3, #4, #5to final mobile phase #6 generates five peaks. After completing theloading by the same earlier illustration of differential set-up betweentwo phases in loading zone 14 to obtain the maximum adsorptionefficiency. All subsequent elution liquid's conditions are predeterminedwith earlier teaching of iso-point belief. The #2 condition has beenpreset prior to the iso-point of desired product Al such that allimpurities are grouped, as B1 will be eluted before product A1. Thiscovers first impurity-stripping step proceeds in firstimpurity-stripping zone 15. The #3 mobile phase has its condition justelutes the desired product A1 prior to iso-point of impurities group B2.This covers the first product-recovery step proceeding in firstproduct-recovery zone 16. The #4 condition is input to cover elution ofimpurities B2 as second impurity-stripping step proceeding in secondimpurity-stripping zone 17. The #5 condition is input to cover elutionof desired product A2 prior to iso-point of impurity group B3. Thiscovers the second product recovery carrying out in secondproduct-recovery zone 18. The #6 condition is input to cover elution of3^(rd)-impurity step proceeding in 3^(rd)-impurity-stripping zone 19.Finally, the washing step is proceeded in washing zone 20 by recyclingof #1 mobile phase collected from feed zone to prepare resin ready forloading again.

The input I-I is illustrated by starting from loading step to obtain themaximum adsorption efficiency proceeded in feeding zone 14 with #1mobile phase. The first impurity-stripping step is followed andproceeded in first impurity stripping zone 15 by minor increment ofmobile phase parameter starting from #1 then ending by #2 to maximizeelution of B1 and avoid the co-elution with product A1. The firstproduct recovery step is proceeded in first product recovery zone 16 byinput I-I starting #2 then ending #3 to maximize the elution of productA1 and avoid co-elution of second impurity group B2. The secondimpurity-stripping step is proceeded in second impurity stripping zone17 by input I-I starting from #3 then ending by #4 for maximizing theelution of second impurity group B2 and avoid co-elution of secondproduct A2. The second product recovery step is proceeded in secondproduct recovery zone 18 by input I-I starting #4 then ending #5 formaximizing the elution of product A2 and avoid co-elution of thirdimpurity group B3. Once the product A2 is eluted, the regeneration stepis proceeded in regeneration zone 19 by input S-I #6 mobile phase tostrip third impurity group B3. Generally, one increment of mobile phaseparameter is sufficient for each increment of minor interval Δt, and yetrequires experimental validation from the target system. Finally, thewashing step is proceeded in washing zone 20 by input S-I with #1 mobilephase recycled from zone 14 to prepare resin ready for zone 14adsorption.

The annular ring shown in FIG. 8 symbolize the simplified apparatus toimplement the differential set-up between two phases from characteristicelution profile described in FIG. 7. It comprises feeding zone 14, firstimpurity stripping zone 15, first product recovery zone 16, secondimpurity stripping zone 17, second product recovery zone 18,regeneration zone 19, and washing zone 20, respectively. The time spentfor each corresponding zone, obtained from last figure is divided bysame minimal time interval Δt in feed zone to gain the number of optimalsteps for such zone. The number of optimal steps is equivalent to numberof cells or groups of cells as one advance step installed in eachcorresponding zone. The resin quantity 124 installed in each cell ofapparatus is equivalent to mass transfer zone 103 in chromatography.Such zone represented by resin 124 is saturated with fixed feed volume123 proceeded in accordance with new mass transfer method.

The time spent for feed loading, under criterion of maximum adsorptionefficiency, divided by minimal time interval to obtain the number ofsteps is the number of cells or group of cells as an advanced step inloading zone 14. After volume 123 divided with number of optimal steps,the pairs of partial feed volume 125 and amount of solid phase 124 isgrouped to constitute as a cell. The input type for zone 14 is inputS-I. The integration of all cells with time allows one cell toaccomplish loading within every spent of minimal time interval. As allcells simultaneously receive the volume 125 and always has one cell or agroup of cells as an advanced step moves out from zone 14 to zone 15.

In first impurity stripping zone 15, the liquid volume 126 representsthe total volume for input S-I of condition #2 or the cumulative volumefor input I-I of liquids between #1 and #2. After volume 126 dividedwith number of optimal steps, the pair of partial volume 127 and resin124 is grouped to constitute as a cell. The integration of all cells inzone 15 accomplishes the elution of first impurity group within everytime interval Δt spent. As all cells simultaneously receive the volume127 and always has one cell or a group of cells as an advanced stepmoves out from zone 15 to zone 16.

In first product recovery zone 16, the liquid volume 128 represents thetotal volume for input S-I of condition #3 or the cumulative volume forinput I-I of liquids between #2 and #3. After volume 128 divided withnumber of optimal steps, the pair of partial volume 129 and resin 124 isgrouped to constitute as a cell. The integration of all cells in zone 16accomplishes the elution of product A1 within time interval Δt spent. Asthe all cells simultaneously receive the volume 129 and always has onecell or a group of cells as an advanced step moves out from zone 16 tozone 17.

In second impurity stripping zone 17, the liquid volume 130 representsthe total volume for input S-I of condition #4 or the cumulative volumefor input I-I of liquids between #3 and #4. After volume 130 dividedwith number of optimal steps, the pair of partial volume 131 and resin124 is grouped to constitute as a cell. The integration of all cells inzone 17 accomplishes the elution of second impurity group B2 within timeinterval Δt spent. As the all cells simultaneously receive the volume131 and always has one cell or a group of cells as an advanced stepmoves out from zone 17 to zone 18.

In second product recovery zone 18, the liquid volume 132 represents thetotal volume for input S-I of condition #5 or the cumulative volume forinput I-I of liquids between #4 and #5. After volume 132 divided withnumber of optimal steps, the pair of partial volume 133 and resin 124 isgrouped to constitute as a cell. The integration of all cells in zone 18accomplishes the elution of second product A2 within time interval Δtspent. As the all cells simultaneously receive the volume 133 and alwayshas one cell or a group of cells as an advanced step moves out from zone18 to zone 19.

In regeneration zone 19, the liquid volume 134 represents the totalvolume of mobile phase #6 for input S-I. After volume 134 divided by thenumber of optimal steps, the pair of partial volume 135 and resin 124 isgrouped to constitute as a cell. The integration of all cells in zone 19accomplishes the elution of third impurity group within every timeinterval Δt spent. As all cells simultaneously receive the volume 135and always has one cell or a group of cells as an advanced step movesout from zone 19 to zone 20.

In washing zone 20, the type of input is input S-I and liquid volume 136of mobile phase #1 can be recycled from fluid collected in zone 14.After volume 136 divided by the number of optimal steps, the pair ofpartial volume 137 and resin 124 is grouped to constitute as a cell. Theintegration of all cells in zone 20 accomplishes the adjusting resinready for loading within every time interval Δt. Because the all cellssimultaneously receive the volume 137 and always has one cell or a groupof cells as an advanced step moves out from zone 20 to zone 14.

In cases of either single or multiple products recovery, each partialliquid volume simultaneously inputs for each cell in various zonesspecifically proceeding one task. The resin installed in each cell iscorresponding to the mass transfer zone 103 in chromatography. Theminimal interval Δt defined in this invention is the time required forspecific liquid to travel through the cell compared with that for masstransfer zone in chromatography. The chromatography operation needs topush such mass transfer zone slowly from one end to emerge from otherend of column to obtain separation. Moreover, the resin loaded in eachcell located in various zones is always active and independent from eachother. From the viewpoint of entire separation cycle, it evidently showsthat the integration of all cells in various zones simultaneously coversstages from loading throughout regeneration and washing for everyminimal interval Δt spent. With employment of disclosed methods ontosaid apparatus, based on same throughput between chromatography and thisinvention, it has successfully demonstrated for reduction of cycle timefrom hours to minutes. It means that the resin inventory can beproportionally reduced as dramatic cycle time reduction. It thenadvantageously reduces the cost of production due resin reduction thatgives rise to smaller equipment to generate numerous economic benefits.

The differential set-up between two phases proceeded through newmass-transfer method enables the process to obtain a swift and precisemass-transfer contact between predetermined mobile phase and resin. Thisspecific mass transfer is achieved in each independent cell and theintegration and coordination of all cells becomes a complete separationprocess. It means the native engineering drawbacks of conventionalcolumn process, such as high-pressure operation and axial dispersion andend effects will not deteriorate the efficiency of new separationprocess. It then concludes that the disclosed process can provide themaximum operation efficiency. This operation efficiency is only limitedby the mechanical capability of preferred apparatus to obtain theexpected separation results. It provides evidence that the processoperation efficiency can be preserved and controlled by process itselfinasmuch as the predetermined mobile phase parameters are suitable. Itmeans the impurity-stripping zone will maximize its stripping efficiencyto assure high throughput of impurities. It means for product recoveryzone, located after the impurity-stripping zone along the processrotation direction, will maximize the production rate, product yield andeconomic efficiency. It means for regeneration and washing zone, locatedafter the product recovery zone and before the feeding zone along theprocess rotation direction, will minimize the process time and maximizethe production rate.

In general, the scale up of existing process from bench top toproduction scale is to quantify the increment of capacity requirements.The production scale simply magnifies with size increments to a largerprocess size. This invention has revolutionized and evidently changedthe rule that simply by increasing the number of bench-top scale to meetsuch capacity requirements. As all cells in said apparatus representingas one small scale that are independent from each other andsimultaneously, perform one task at any instance such that theintegration and coordination of all cells become a complete process.Particularly, unit chemical operation composes of sequential stages thatare linked together to carry out such operation. The traditionalscale-up strategy focuses mainly on size increment and often ignores thecoordination that part of stages may be idled during the-proceeding ofentire operation. Particularly in column operation been long recognizedfor inefficient usage of packing materials, this invention has clearlyratified the strategy of scale up by implementation of differentialmethod between two phases and new mass transfer method. The preferredapparatus has demonstrated the mechanical capability to implement andtransform the path of the mass transfer from sequentially verticaldirection to simultaneously horizontal direction. Both methods and meansto implement such methods are tied together as hybrid embodiments. Thesehybrid embodiments can be extended to other resembling chemical unitoperation such as catalytic reactions in packed bed and ordinaryfluidized reaction involved in solid and liquid phases. The capabilitiesof cycle time reduction via differential method and preferred apparatuscan benefit reduction of process size proportionally and ends up withproduction cost reduction.

The principle objects of the present invention is to provide acorresponding operation protocol for preferred apparatus to implementthe proposed methods; to continuously and automatically isolate a singleproduct or multiply products from a target mixtures. Only single productcase by input S-I mode will be illustrated for sake of simplifieddiscussion. For the input I-I mode needs to further break down theliquid delivery module for each cell for different liquid input. Asshown in FIG. 1, all vacuum pumps are necessary to be employed the wholetime to continuously drain the liquid from all cells and to maintainresin in semi-dry status for the criterion of said new mass transfermethod. Liquid input is conducted as described in FIG. 3 as said newmass transfer method to promote designated mass transfer. Moreover, itshall understand these two cells as a group, as an advanced step isarbitrary for demonstration only. The protocol comprises of three stagesof operation method, including startup stage, steady state stage, andtermination stage.

The startup stage beginning from first group of two cells 11 located atbeginning position in zone 14, the predetermined amount of feed solutionis delivered via a solenoid valve 54 located above these cells. Theduration of valve opening is preset to control the volume of feeddelivery. Soon after the feed delivery is completed, all solenoid valves55 are engaged to purge the pressure air or inert gas to affiliate thevacuum to settle the bed, to percolate the #1 liquid into compartment20. All valves 55 are de-energized to shut off pressure air. This bringsthe fluid delivery to end. The center-mounted motor 89 is energized torotate and terminated one step forward by said rotation and positioningmechanism. Simultaneously during the rotation, the liquid solenoid valve44 (not shown) is opened to guide the collected liquid in compartment 20into underneath holding tank. The whole sequences of movement accomplishwithin one minimal time interval, Δt. This brings the first cells intosecond position, following cells from last position of zone 18 intobeginning position of zone 14. Then, the first and second solenoidvalves 54 are simultaneously opened to deliver prefixed volume of feedsolution into each underneath cell. Soon after both liquid valves 54 areshut off, all air valves 55 are opened to purge air to settle the bedand drain the liquid into compartment 20. Then, all air valves 55 areshut off. The motor 89 is energized again to rotate and meantime toengage liquid valve to guide liquid into underneath holding tank 25; andthen terminate one advance step forward. This whole sequence of movementis completed by same minimal time interval, Δt. Note only two steps arerequired in zone 14; means the resin retained in second step has alreadysaturated with feed solution. The third rotation will move these cellsoriginal at beginning position out of zone 14 into first position ofzone 15. This brings meanwhile the cells in beginning position intosecond position of zone 14 and following cells into first position ofzone 14. The first valve 54 in zone 15 and two valves 54 in zone 14 aresimultaneously opened. The cells in first position of zone 15 receives#2 mobile phase that can elute first impurity group, meanwhile loadingis proceeding for both steps in zone 14. After the prefixed mobilephases finished delivery, all air valves 54 are opened to release air tosettle the beds and drain the liquids into compartment 20 in zone 14 andcompartment 21 in zone 15 to end. The motor 89 is engaged again torotate and simultaneously drain the liquid into respective holding tank.Again, these procedures spent by same minimal time interval. Note thosetwo steps in loading zone and total twenty-two steps covering all zonesare arbitrary picked for easy illustration, and it does not reflect toany specific separation system.

The cycles of concurrent liquids input up to where the first celllocated, pressure air or gas releasing, bed settling with liquiddraining, pressure air or gas shutting off, rotating one step forwardand simultaneously guiding liquid through liquid solenoid valve intoholding tank are repeated repeatedly. Since the time spent for therepeated cycle is same for each step and is tied in with thecharacteristic profile of target separation system; in which are relatedwith said illustration in FIG. 5 through FIG. 8. Thus, the first cellstakes total twenty-two steps, by starting from the beginning position inzone 14 throughout the last position in zone 18 to complete theirjourney as a complete separation cycle. These particular cells return totheir beginning position on the twenty-thirds step. The whole separationcycle of particular system is proceeded under the criterion of new masstransfer method via differential set-up between two phases onto thepreferred apparatus.

After the first cell initially at beginning position of zone 14completes the last position in zone 18 and return to its initialposition. This is the beginning of steady-state stage. It means allcells in various zones will simultaneously receive the various fluids toproceed operation of loading, impurity stripping, product recovery,regeneration, and washing. The repeated cycles of liquid input topartially fluidize the bed, releasing of pressure air to settle the bedand drain the fluid, pressure air shut off, and advance one rotationstep are simultaneously proceeded for all cells in all zones. All cellsare independent from each other and combination of all functions of allcells becomes a complete separation cycle. It means that every spent ofminimal time interval at steady state stage, Δt, is for accomplishing acomplete separation cycle concurrently covering from loading throughoutwashing. The predetermined characteristic elution is the outcome of newmass transfer contact method and differential set-up between two phases,which are implemented by the steady-state operation of the preferredapparatus during every spent of minimal time interval, Δt.

The termination stage provides the proper procedures to terminate theproceeding of steady-state stage. It allows the resin retained in eachcell for all zones returns to fresh status. As shown again in FIG. 1,once the termination stage is engaged. The repeated cycle of liquidinput is stopped from the beginning position of loading zone 14 by notengaging the liquid valve 54 located above these cells. Other positionsin the apparatus still simultaneously proceed the same repeated cyclesby every minor time interval. As the cells located at first position ofzone 14 comes from the last position of zone 18, which retain the resinat fresh status. After the first spent of Δt, the cells initially at thefirst position do not receive the feed move to second positions in zone14. The following cells from zone 18 takes beginning position in zone14, which are fresh resin as well. After the second spent of Δt, bothfirst and second position in zone 14 maintains fresh resin and othercells repeat same repeated cycles of liquid filling and so on. Therepeated stop liquid input is stepwise to include the next position ofcells until the cells initially located at beginning position of zone 14move into the last position of zone 18. Particularly shown in FIG. 1,this termination stage takes same twenty-two steps as that in startupstage to complete. This will conclude the termination stage; meaning theresin retained in all cells is in fresh status. Finally, all vacuumpumps installed in various zones can be terminated as well.

Both of FIG. 9 and of FIG. 10 exhibit the simplified disclosed apparatusby an annular ring divided in part for representing various zones. TheFIG. 9 shows the single stage recycles for single product recovery, asto previous illustration in FIGS. 6 and 7. The schematic proceduresemphasize the fluid flow after solid and liquid contact fordemonstrating the concurrent separation, efficient usage of mobilephases, and concentration enhancement of separated fractions. Althoughin reality, it may require simultaneously recovery of multiple products.However, this shall serve the purpose of illustration for generalrecycle protocol. The various streams of fluid being drained andcollected to each corresponding holding tank, which can be recycled backto same zone or different zones as required from the target system. Thenumber of cycles of fluid being recycled for each zone is varying andmay require experimental evaluation prior the employment of recycleprotocol.

Soon after the startup stage is finished, all zones are proceededsimultaneously for steady state operation. The feed input via line 138and other fluids are simultaneously delivered to all cells in all zones.It means the collected fluid from the zone 14 and is temporally storedin tank 25 via line 139. The fluid is delivered from tank 25 via line140 into zone 18 for economic usage of mobile phase to adjust the resinto fresh status ready for feed zone 14. It means the collected fluidfrom zone 18 via line 141 stored in tank 29 can be discarded ordelivered for further treatment via line 142. This fluid flow circuit isconstantly maintained for startup stage, steady state stage, andtermination stage.

The recycle circle is concurrently maintained in zone of 15, 16, and 17during the steady state operation. It means that the predetermined freshfluid #2 is delivered into first impurity stripping zone 15 via line300, while line 144 is shut off. The collected fluid from this zone vialine 143 and is temporally stored in holding tank 26. It means that thepredetermined fresh fluid #3 is delivered into the product recovery zone16 via line 301 while line 146 is shut off. The collected fluid fromthis zone via line 145 and is temporally stored in tank 27. It meansthat the predetermined fresh fluid #4 is delivered into regenerationzone 17 via line 302, while line 148 is shut off. The collected fluidfrom this zone via line 147 and is temporally stored in tank 28.

Once the fluid collected in each holding tank is sufficient forsimultaneously recycling. The fluid is recycled from tank 26 via line144, while line 300 is shut off, and delivered back to the same zone forthe economic saving to intensify the impurity concentration level withinthe same zone. The fluid is recycled from tank 27 via line 146, whileline 301 is shut off, and delivered back to the same zone for economicsaving and enhancing the product concentration level within the samezone. The fluid is recycled from tank 28 via line 148 while line 302 isshut off, and delivered back to the same zone for economic savings andintensifying the concentration level of second-impurity group within thesame zone. Note that while the recycle protocols are proceedingconcurrently in zones 15, 16, and 17, the loading and washing areproceeding simultaneously as well in zones 14 and 18.

After spending the predetermined number of cycles, the fluid can besimultaneously discarded until loses its activity to perform itsfunction or to be recovered as concentrated product for designatedconcentration level. It means the fluid been recycled in zone 15 can bedelivered from tank 26 via 149, while line 144 is shut off, to bediscarded or delivered further treatment. It means the fluid beenrecycled in zone 16 can be delivered from tank 27 via 150, while line146 is shut off, to be recovered as concentrated product. It means thefluid been recycled in zone 17 can be delivered from tank 28 via 151,while line 148 is shut off, to be discarded or delivered furthertreatment. Then, fresh fluids for zones 15, 16, and, 17 can besimultaneously reintroduced to make up fluids been removed. The fresh #2fluid can be delivered via line 300, while line 144 is shut off, toproceed the separation and to make up the removal. The fresh #3 can bedelivered via line 301, while line 146 is shut off, to proceedseparation and to make up the removal. The fresh #4 can be delivered vialine 302, while line 148 is shut off, to proceed separation and to makeup the removal. Once, the make up of fluid for each zone isaccomplished, the recycle can be resumed until another set of recyclesare spent. By doing so, the efficient usage of various mobile phases indifferent zone can be maximized.

The termination protocol for single stage recycle to allow the processto return to its initial status from the steady state operation, whichis same as the earlier illustration of termination stage. Preferably,the last cycle of separation is set with recycle mode due all cells willpass through washing zone 18 that resin packed in each cell can bewashed prior the completion of termination stage.

FIG. 10 explicates the multiple stages recycle by serving five unitsconnected in series with single product recovery in accordance with FIG.6 to simplify the demonstration. For a particular target system, thenumber of stages is the number of apparatus connected in series and isequal to the predetermined number of cycles said in the single stageillustration. In general, the feed throughput is evenly divided by thenumber of stages in a series. Such partial amount of feed throughput andthe zone establishment for each corresponding stage is transmittedthrough each characteristic elution profile derived from the said newmass transfer method and differential set-up between two phases.Moreover, the path of distribution the fluid from current stage to nextstage can be varied with different target system. It may be distributedas input for the designated zone in next stage, or as partialdistribution within the same stage and partial distribution as input forthe designated zone in next stage. It may be reserved as product orby-product for particular stage and partially distribute for next stageand fresh liquid needs to make up in the next stage for fluid has beenreserved as product or by-product in previous stage. The purpose ofrecycle is for utilizing mobile phase in a most efficient manner tosimultaneously obtain separation and concentration enhancement ofseparated fractions. Thus, the operation protocol for multiple recyclestages, including start-up step, steady-state step, and terminationstep, is exemplified via liquid flow path indicated in FIG. 10.

The start-up step for multiple recycle stages is the sequentialapplication of aforementioned startup stage for individual unitconnected in series. While the remaining four units are idled, the firstunit starts feed input via line 152 shown as Stage 1 in FIG. 10 throughprocedures of startup stage. Such that the first cell initial atbeginning position completed the journey to return to its initialbeginning position in zone 14. At this moment of completing of startupstage, the feed input need to be started in unit #2 via line 153simultaneously with the steady-state proceeding of unit #1.

During the startup stage of unit #1, the holding tank 25 has alreadydelivered the fluid collected from zone 14 via line 154 into zone 18 vialine 155. The collected fluid via line 156 temporally stored in tank 29may be discarded or delivered for further treatment via line 157. Thefresh #2 mobile phase is initially delivered via line 303 into zone 15and the holding tank 26 has collected sufficient fluid via line 158 andrecycled as an input via line 159 into zone 15 of unit #2. The fresh #3mobile phase is initially delivered via line 304 into zone-16 and theholding tank 27 has collected sufficient fluid via line 160 and recycledas an input via line 161 into zone 16 of unit #2. The fresh #4 mobilephase is initially delivered via line 305 into zone 17 and the holdingtank 28 has collected sufficient fluid via line 62 and recycled as aninput via line 163 into zone 17 of unit #2. The collected fluid via line164 from zone 14 of unit #2 into tank 25 is delivered via line 165 tozone 18 to adjust the resin for adsorption. The collected fluid via line166 into tank 29 of unit #2 may be discarded or delivered for furthertreatment via line 167. The timing and sequence of recycled fluidsdelivered from unit #1 into various zones in unit #2 are preset throughaforementioned startup stage protocol.

Soon after the startup stage for unit #2 is completed, the third unit inseries then engages its startup stage by feed input via line 168 whileunit #4 and #5 remains idle. It means the fresh fluid concurrentlydelivered via lines 303, 304, and 305 into zones 15, 16, and 17, then,collected and simultaneously delivered via lines 159, 161, and 163 intocorresponding zones 15, 16, and 17 of unit #2. Meanwhile, the fluidcollected from zone 15, 16, and 17 in unit #2 is sequentially deliveredat appropriate timing through aforementioned startup protocol to supportthe fluid required for startup stage of unit #3. It means the fluidcollected via line 169 from zone 15 of unit #2 is temporally stored intank 26 and recycled as input via line 170 to zone 15 of unit #3. Itmeans the fluid collected via line 171 from zone 16 in unit #2 istemporally stored in tank 27 and recycled as input via line 172 to zone16 of unit #3. It means the fluid collected via line 173 from zone 17 inunit #2 is temporally stored in tank 28 and recycled as input via line174 to zone 17 of unit #3. The fluid collected via line 175 from loadingzone 14 and temporally stored in tank 25 is delivered via line 176 intozone 18 for resin adjustment. The collected fluid via line 177 into tank29 may be delivered via line 178 for further treatment. Again, all cellsin unit #3 stepwise rotate through aforementioned startup protocol toreceive various fluids to allow the first cell initial in beginningposition of zone 14 to return its initial position.

Soon after the startup stage for unit #3 is completed, the fourth unitin series engages the startup stage by feed input via line 179. Whilethe first through third unit simultaneously proceeds the steady-stateoperation and fifth unit remains idle. Likewise, the fluid collectedfrom first and then through second unit are simultaneously deliveredfrom holding tanks via lines 170, 172, and 174 into corresponding zones15, 16, and 17 of unit #3. Meanwhile, the fluid collected from zone 15,16, and 17 in unit #3 is sequentially delivered at appropriate timingthrough aforementioned startup protocol to support the fluid requiredfor startup stage of fourth unit. It means the fluid collected via line179 from zone 15 in unit #3 is temporally stored in tank 26 and recycledvia line 180 as input into zone 15 of unit #4. It means the fluidcollected via line 181 from zone 16 in unit #3 is temporally stored intank 27 and recycled via line 182 as input into zone 16 of unit #4. Itmeans the fluid collected via line 183 from zone 17 in unit #3 istemporally stored in tank 28 and recycled via line 184 as input intozone 17 of unit #4. The fluid collected via line 185 from loading zone14 in unit #4 and temporally stored in tank 25 is delivered via line 186into zone 18 for resin adjustment. The collected fluid via line 187 intotank 29 may be delivered via line 188 for further treatment. Again, allcells in unit #4 stepwise rotate through aforementioned startup protocolto receive various fluids to allow the first cell initial at beginningposition of zone 14 to return its initial position.

Soon after the startup stage for unit #4 is completed, the fifth unit inseries will engage the start-up stage by feed input via line 189 whilethe first through fourth unit simultaneously proceeds the steady-stateoperation. Likewise, the fluid collected from the first through thirdunit in series are simultaneously delivered via lines 180, 182, and 184into corresponding zones 15, 16, and 17 of unit #4. Meanwhile, the fluidcollected from zone 15, 16, and 17 in unit #4 is sequentially deliveredat appropriate timing through aforementioned startup protocol to supportthe fluid required for startup stage. It means the fluid collected vialine 190 from zone 15 in unit #4 is temporally stored in tank 26 andrecycled via line 191 as input into zone 15 of unit #5. It means thefluid collected via line 192 from zone 16 in unit #4 is temporallystored in tank 27 and recycled via line 193 as input into zone 16 ofunit #5. It means the fluid collected via line 194 from zone 17 in unit#4 is temporally stored in tank 28 and recycled via line 195 fromholding tanks into zone 17 of unit #5. The fluid collected via line 196from loading zone 14 in unit #5 and temp rally stored in tank 25 isdelivered via line 197 into zone 18 for resin adjustment. The collectedfluid via line 198 into tank 29 may be delivered via line 199 forfurther treatment. Again, all cells in unit #5 rotate forward throughaforementioned startup protocol to receive various fluids to allow thefirst cell at beginning position of zone 14 to return its initialposition. This concludes the startup stage for unit #5 and brings to anend for the start-up step.

The process is ready for steady-state step; meaning each unit,exemplified in series of five, is in steady state operation. The zone 14of unit #1 through #5 simultaneously undergo partial feed input vialines of 152, 153, 168, 179, and 189. Simultaneously collecting fluidfrom zone 14 via lines of 154, 164, 175, 185, and 196 and temporallystore in each holding tank 25; and concurrently deliver via lines of155, 165, 176, 186, and 197 into zone 18. The fluids are furthercollected via lines of 156, 166, 177, 187, and 198 from each zone 18into corresponding tank 29 and simultaneously delivered via lines of157, 167, 178, 188, and 199 for further treatment or discarded. Thefluid flow from each zone 14 toward zone 18 located in same stage is forresin adjustment prior forwarding into loading zone.

Under the steady state operation, the zone 15 of first unit receivesfresh #2 mobile phase via line 303. Simultaneously, the used #2 mobilephase is flowed from each intermediate holding tank 26 of first unitthrough fourth unit via lines of 159, 170, 180, and 191 into respectivezone 15 of the following unit. Simultaneously, each intermediate holdingtank 26 receives the collected fluid from the corresponding zone 15 vialines of 158, 169 179, 190, and 200. Moreover, the used mobile phase,enriched in first impurity group, is simultaneously delivered via line201 for further treatment.

Simultaneously, the zone 16 of first unit receives the fresh #3 mobilephase via line 304. Simultaneously, the used #3 mobile phase is flowedfrom each intermediate holding tank 27 of first unit through fourth unitvia lines of 161, 172, 182, and 193 into respective zone 16 of thefollowing unit. Simultaneously, each intermediate holding tank 27receives the collected fluid from the corresponding zone 16 via lines of160, 171, 181, 192, and 202. Moreover, the used mobile phase, enrichedin desired product, is simultaneously delivered via line 203 for furthertreatment.

Simultaneously, the zone 17 of first unit receives the fresh #4 mobilephase via line 305. Simultaneously, the used #4 mobile phase is flowedfrom each intermediate holding tank 28 of first unit through fourth unitvia lines of 163, 174, 184, and 195 into respective zone 18 of thefollowing unit. Simultaneously, each intermediate holding tank 28receives the collected fluid from the corresponding zone 17 via lines of162, 173, 183, 194, and 204. Moreover, the used mobile phase, enrichedin second impurity group, is simultaneously delivered via line 205 forfurther treatment.

As the solid phase retained in each cell of respective stage representsas a mass transfer zone, of which rotates in horizontal direction toperpendicularly contact with mobile phases in partially fluidized basis.For the synchronized rotation of all cells in respective stage ofmultiple-stage recycle protocol, the process is providing a steady flowof mobile phases in perpendicular direction to intermediately interactwith the solid phase moving in horizontal direction. Throughimplementation of new mass transfer method and differential set-upbetween two phases, the current invention simulates the characteristicelution profile of each stage and integrates with the preferredapparatus. It transforms the traditional sequential chromatographyprotocols to continuous and concurrent protocols. Thus, both concurrentseparations of components from the target mixtures and enriching theconcentration level of the separated components are simultaneouslyaccomplished through continuous execution of these protocols. Moreover,the protocols for multiple-stage recycle have carried out the multiplemass transfer tasks simultaneously from loading to washing for eachstage within every spent of minimal time interval, Δt. At any instanceof steady state operation, this invention is permissive throughfulfilling of these protocols to allow for the usage of both phasesinvolved in mass transfer contact in an extreme flexibility and mostefficient manner.

The termination step is to bring the process to return to its initialfresh status from steady-state operation. It proceeds the same order asin start-up step to sequentially employ the termination procedures bystarting from first unit while remaining units still maintain thesteady-state operation. The termination of particular unit in seriesfollows the same procedures of aforementioned termination stage. Soonafter the first unit becomes idle through completing the terminationstage, the second unit starts engaging its termination procedures whilethe remaining units are still at steady-state proceeding. Then, thirdunit begins its status for termination after first and third unit becomeidle while the remaining units are still at steady-state operation. Thestage of sequential termination applied toward the last unit to completefinal termination stage for such unit and so is the conclusion oftermination step protocols for multiple stages of recycle.

Following is an illustrative example to demonstrate the fact ofiso-point as fundamentals for establishing elution protocols. Then, twodistinctive elution profiles, between traditional chromatography and newmass transfer method, of high fructose-corn-syrup, abbreviated as HFCS,are shown. Finally, four sets of elution profiles of same sugar mixturesare shown to illustrate the recycle protocols. The new mass transfermethod and differential set-up between two phases are employed in therecycle protocols for effective usage of mobile phase and dramaticreduction of cycle time. As with the reduction of cycle time, it reducesthe resin stock based on it feed throughput compared between thedisclosed process and traditional chromatography.

EXAMPLE 1

In this example, a single component protein solution, hemoglobinabbreviated as Hm, is explored on traditional chromatography todemonstrate the iso-point belief by cyclic adsorption and desorption. Hmhas molecular weight of 63,000 and iso-electric point is at pH6.7. Theprotein will carry positive charge in solution with pH lower than 6.7and conversely carry a negative charge in solution with pH higher than6.7. The chromatography column has 2.6 cm in I.D. and 15 cm in length,was fully loaded with CM Sepharose; a cation exchanger. The Hm feedsolution was prepared at 0.01 wt. % in 0.1M Tris-HCl buffer solution.The reciprocating flow through the column was achieved by a reversibleperistaltic pump, which is connected to a timer for precise measurementof the sample volume. Multi-way valves are installed on column inlet tointroduce the feed and elution buffer at different pH. Each samplestream was collected at equal time intervals for pH and proteinconcentration measurement.

In FIG. 11 shows the cyclic wave of pH curve and Hm concentrationprofile in dimensionless form (Yp/Yo) for each collected sample withrespected to the accumulated elution volume (E.V.), cc. The feedsolution was prepared at pH 6.0 and Hm carries positive charge,oppositely to cation exchanger loaded in column. The introduction of67.5 cc of feed solution by pulse input 106 as described in FIG. 5,causes Hm been adsorbed onto the resin starting from very beginning ofcolumn. This resin is corresponding to zone 103 described in FIG. 5. Theremaining resin, analogous to zone 104, is saturated with pH 6.0 buffersolution. The introduction of 67.5 cc of pH 8.0 buffer by pulse input106 will push the existing pH6 buffer exit from other end of column.Analogous to the narrow transient region 108 illustrated in FIG. 5, thecontrol parameter is progressively changing from pH6 to pH8, in whichthe Hm concentration profile is confined. Each Hm profile has a highestturning and its corresponding pH is 6.7, which is same as itsiso-electric point. Note that the Hm and pH profiles are history ofeluted Hm band travel starting from zone 103 throughout zone 104 andcollected as samples. Initially, it is a very sharp and narrow band andbecome wider as this band approaching toward the column outlet, mainlycaused by the diffusion and axial dispersion. However, some diffused Hmmay enter areas that mobile phase pH is lower than 6.7 and cause the Hmbeen adsorbed again and eluted by upcoming pH higher than 6.7. Thisexplains each Hm profile shown in this figure has flat front located inregion 206 between pH 6.0 and 6.7 while long tail been expanded inregion 207 located after 6.7 toward the end of pH 8.0. This phenomenongets worse in large-scale column and has been tolerated for decades inchromatography related separation process. As earlier discussion, thesenative engineering drawbacks of traditional chromatography operation istime consuming, inefficient usage of resins limited loading capacity,and activate the axial dispersion and column end-effects to ruin theinitial separation, etc. This phenomenon also validates the belief forthe desorption of adsorbed solute components will not occurr unless thesurrounding mobile phase has exceeded this component's iso-point. Bycontrolling the separation parameter and by pass these phenomena towardnew mass transfer method and differential set-up between two phases arethe fundamentals of this disclosure.

EXAMPLE 2

The sugar mixtures, high-fructose-corn-syrup (HFCS) as feed, isinvestigated by traditional chromatography to show the difference fromthe elution profile studied under new mass transfer method in nextexample. The feed solution has 60% dry substance, containing 44.1% offructose, 51.0% glucose, and remaining 4.9% oligosaccharide. The columnis 1.6-cm (I.D.)×40-cm (L) maintained at 75° C. by water circulationthrough column jacket. The resin filled in column is calcium basestrongly acidic cation exchanger with mean particle size of 320 um±10um. The resin filled in column has 80 cc of bed volume. The feed andde-ion water reservoir were jacked with 75° C. water circulation. Thefeed solution and eluent water was fed sequentially via a two-way valvefrom top of column by a reversible peristaltic pump, which is connectedwith a timer for precise 2 cc/min. volume input rate. Samples arecollected at column outlet by every two-minute interval. The column isinitially saturated with de-ion water and first input by four cc of feedsolution, and then eluted by de-ion water, all input at 2 cc/min flowrate.

In FIG. 12, the elution profiles are plotted in D.S.% (dry substance)vs. B.V.% (bed volume) for oligosaccharide; abbreviated as oligos,glucose, and fructose. The sugar components start to elute after 50% ofbed volume and end after onebed volume, a typical characteristic profilefor chromatography. The sugar components are first adsorbed by resin andeluted by upcoming water. Oligos is the least retained ingredient amongthree components, thus eluted first. Followed is the glucose and then byfructose. Similar to the elution profile shown in FIG. 11, the glucoseand fructose both have flat front profile and long tail; indicating theinitial separation were deteriorated significantly by axial dispersionand diffusion. The elution-profile indicates the control parameters forthis system is de-ion water and suggests the iso-point of glucose andfructose is very close to each other. Thus, the amount of water inputfor separation is critical. Note that total eluent water consumed is 88cc that is equivalent to 110% resin bed volume, or consumed water tofeed ratio is 22 for this example.

EXAMPLE 3

The same feed solution of HFCS was investigated under new mass transfermethod to distinguish of mass transfer phenomena between this disclosureand chromatography. The feed and eluent reservoirs and cylindrical cellwith dimension of 1.66-cm (I.D.)×195-cm (L) are jacked with 75° C. hotwater circulation. The same resin, in semi-dry status, was filled with406 cc in volume and having 185 cm in final abed height. As demonstratedin FIG. 3 that an approximate 10-cm open space is reserved between fluidinlet baffle and resin for creating partial fluidize effect. Unlikechromatography, the resin is always saturated with liquid. The semi-drystatus means the resin may have wet surface and inter-particle's fluidis drained. The 21 inch-Hg vacuum is employed from bottom of the cellfor continuous liquid draining. The bottom of the cell is equipped withthread on sample bottle, which is airtight and can be manually threadoff to replace with new sample collection bottle. The fluid input wasmanually delivered by a liquid pipette to simulate the input S-I forboth feed solution and eluent water.

Total feed volume is 40 cc, equivalent to 0.1 bed volume of resin, isdivided into 10 cc/dose via a quick pipette stroke and wait until 30seconds are spent before next 10 cc is delivered. Total four pipettestrokes are delivered within two minutes period, plus additional twominutes vacuum for draining. The fluid collected as sample #1 has 12 ccin volume, as shown in FIG. 13, which is virtually free of glucose,fructose, and only 0.29% D.S. of oligos. This first sample is 99.7% ofwater, which is the exchanged water when sugars are bounded onto theresin in mass transfer zone. This phenomenon is not been realized intraditional chromatography, mainly because the column is initiallysaturated with water and additional exchanged water cause sugarcomponents return to mobile phase. Nevertheless, this is also the majordistinction between this disclosure and chromatography in aspects ofresin's adsorption capacity, which enable the same resin to increase itsbonding capacity three to four folds under new mass transfer method.

After the loading is completed, eluent water is input by several formatsof input S-I and samples are collected by every four minutes. Format #1covers sample #2 to #5. Each water dose delivered is 0.7 cc and idleduntil ten seconds are spent before the next 0.7 cc is delivered. Total 8doses are delivered within two minutes, which includes 40 seconds idledtime. It then repeated with same input format for additional two-minuteperiod. For simple notation, the input S-I can be denoted as ((0.7 cc/10sec.)*8/2 mins)*2 with total 11.6 cc water input for four minutesperiod. Then, no water is delivered for sample #6. Repeat Format#1 forsample #7 and #8. Then, no water is delivered for #9. Format #2 coverssample #10 to #12, which is delivered as ((0.5 cc/10 sec.)*8/2 min)*2for total 8 cc for every four minute period. Then, no water is deliveredfor #13. Same format #2 covers sample #14 to #16. Then, no water isdelivered for #17. Format #3 covers sample #19 to 23, which is deliveredas ((0.9 cc/10 sec.)*10/2 min)*2 for total 18 cc for every four-minuteperiod. Then, no water is delivered for last sample #24. The summationof total water delivered is 229.2 cc, which is equivalent to 56.5% ofresin bed volume, or consumed water to feed ratio is 5.73. Asaforementioned characteristic elution of a single bed transformed inconjunction with the preferred apparatus that one revolution of aparticular cell moving from loading to washing stage is equivalent tosuch elution profile. Thus, this example shall serve as the template,through which the preferred apparatus can be implemented. The majordifferences from chromatography, shown in FIG. 13, are the separationthat starts from the very beginning and ends at 50% of bed volume, andexhibits much better separation between glucose and fructose. Table 1below shows the averages concentration for mixtures of samples 5 to 13and 19 to 24. The recovery of sugar is defined as the-weight of totalsugar output vs. total sugar recovered. Note that the initial sugarweight in feed is 30.624 grams and total sugar recovered is 30.470grams.

TABLE 1 Sample Total Output Recovery % Glucose % Fructose %  5 to 13 7.642 grams 25 91 9 19 to 24 10.880 grams 35.7 8 92

The recycle protocols are illustrated in the following examples fordemonstration of efficient consumption of solid and mobile phase, and toconcentrate the separated fractions while separation are notinterrupted. Four cycles of single elution profiles are shown tosimulate the actual separation cycle observed on preferred apparatus.The same bed used in example 3 is been used again and maintaining insemi-dry status as criterion of new mass transfer method. The hot watercirculated in bed jacket is maintained at 80° C. The vacuum isconstantly engaged at 27 inch-Hg vacuum. In these examples, the watervapor is recovered by passing the exit hot air through a condenser,jacketed with circulated cooling water, to condense the water vaporbefore entering the vacuum pump. All collected water from bottom ofcondenser is free of sugar residues and can be reused. The same feedsolution applied, as input S-I for each cycle is 130 cc, equivalent to0.32 resin bed volume. The eluent-water and recycled streams fromprevious cycle is also been delivered respectively as input S-I. Allliquid input is manually delivered by pipette to simulate the input S-I.Soon after the liquid dose is delivered, the pressure air, about100-psi, was released manually from the top of cell to affiliate thevacuum for quick liquid draining. In each run of four elution profilesdescribed as template for apparatus; the number of steps, in accordancewith the differential set-up between two phases, are 45 steps and eachstep or minimal time interval been counted for 30 seconds.

EXAMPLE 4

For illustration of recycle protocol demonstrated in FIG. 9 and FIG. 10,the elution profile shown in FIG. 14 for separation of HFCS indicates asthe first cycle in series of total four cycles. The input S-I forloading covers from sample #1 through #7. Sample #1 is by inputting 20cc of feed and followed by pressure air to make up first 15 seconds.Then, it repeats additional 20 cc in next 15 seconds plus 90 secondspressure air to make up total 40 cc feed in two-minute loading time. Itcan be denoted as ((20 cc/15 sec.)*2)*4, meaning this sample has 40 ccfeed and counts for four minimal-time-intervals or, for 30 seconds eachstep. Total 24 cc of exchanged water is collected as sample #1 withsimilar results as for sample #1 in last example. Sample #2 is done by((5 cc/5 sec.)*3)*1; meaning three of (5 cc/5 sec.) feed plus 15 secondspressure air to count as one step for 30 seconds. Sample #3 is done by((5 cc/5 sec.)*3)*3, which is counted as three steps for 90 seconds. Thesample #2 and #3 counts for total four steps as one format. Thefour-step format is repeated for sample #4 and #5, and repeated forsample #6 and #7. Each group is counted as four steps. This concludesthe total 16 steps of loading for total 130 cc of feed solution, whichcontains 99.528 grams of dry base sugar.

The eluent water starts from sample #8 to #31 with the same format ofinput S-I as ((4 cc/5 sec.)*3)*1, means each collected sample is countedas one step for 30 seconds, each having 12 cc of water input. Exceptsample #9 is ((4 cc/5sec.)*3)*3 for three steps. The complete water onhas twenty-six steps with total 288 cc-water consumption. The samples#32 through #34 were collected by pressure air for one step per sample,to drain the remaining liquid and prepare the bed for next cycle. Thecondensed water collected from condenser is 36 cc to make net of 252cc-water consumption. The table 2 shows the group of sample mixturesclassified as zone 1 through zone 6 to cover samples #1 through #34. Themixtures of zone 2 and zone 3 will be considered as by-productabbreviated as BP, namely glucose enriched solution and fructoseenriched solution. The mixture solutions in zone 4,5,6, and 1 will berecycled into the second cycle.

TABLE 2 Zone Sample No. D.S. % Oligos % Glucose % Fructose % Volume, ccSugar, gm 1 1-8 8.04 22.45 59.62 17.93 72 5.9555 2 9-16, BP 40.13 7.0258.9 34.08 54 25.1783 3 17-20, BP 53.05 0 43.95 56.05 43 27.9737 4 21-2541.97 0 29.24 70.76 50.3 24.7209 5 26-30 17.68 0 13.08 86.92 55 10.36096 31-34 3.21 0 0 100 29.5 0.9577

EXAMPLE 5

The elution profile of HFCS shown in FIG. 15 demonstrates the secondcycle of recycle protocols. The input S-I format for loading stage forsample #1 is ((35 cc/15 sec.)*2)*4, which counts for four steps to inputtotal 70 cc feed solution and collects 33 cc of exchanged water assample #1. The sample #2 and #3 follow identical format as those inexample 4, which are ((5 cc/5 sec)*3)*1 and ((5 cc/5 sec)*3)*3 to becounted as four-step format. The sample #4 and #5 repeat same four-stepformat to count for 30 cc feed. The loading stage delivers total 130 ccfeed and spends six minutes for twelve steps or twelve minimal-timeintervals.

The elution starts from the recycle stream of mixture(#21-25) of zone 4shown in Table 2. The input S-I format for sample #6 is ((4.5 cc/5sec)*3)*1 to count for one step of total 13.5 cc recycled solution. Thesample #7 is ((4.5 cc/5 sec)*3)*3 to count for three steps. The sample#8 is ((4.5 cc/5 sec)*3)*1 for one step. The sample #9 is (4.5 cc/5sec)*2 of solution from zone 4 and (4.5 cc/5 sec)*1 from zone 5 to makeup total one step, due the solution:from zone 4 is finished. However, inactual operation of the preferred apparatus, this will not be happened.As enough solution will be accumulated in each zone's holding tank forcycling into next cycle. The sample #10 is ((4.5 cc/5 sec)*3)*1 for onestep of total 13.5 cc recycle solution from zone 5. Samples #11 athrough #12 are ((4.5 cc/5 sec)*3)*1 for each one step to total twosteps. The sample #13 is (4.5 cc/5 sec)*2 of recycle from zone 5, duethe solution is finished, plus (4.5 cc/5 sec)*1 from zone 6 to make uptotal one step of total 13.5 cc recycle solution. Samples #14 and #15each has one step of recycle solution from zone 6. Samples #16 through27 are ((4.5 cc/5 sec)*3)*1, each one has one step of 13.5 cc eluentwater. The eluent water has total twelve steps to consume 162 cc involume. Samples 28 through 32 are recycle solution from zone 1 ofprevious cycle, each one is ((4.5 cc/5 sec)*3)*1 of 13.5 cc solution.The samples #33 through #35 were collected by pressure air for one stepto drain the remaining liquid. The condensed water collected fromcondenser is 27 cc to make net of 135 cc water-consumption. The table 3shows the group of sample mixtures classified as zone 1 through zone 6to cover samples #1 through #35. The mixtures of zone 2 and zone 3 willbe considered as by-product abbreviated as BP, namely glucose enrichedsolution and fructose enriched solution. The mixture solutions in zone4,5,6, and 1 will be recycled into the third cycle.

TABLE 3 Zone Sample No. D.S. % Oligos % Glucose % Fructose % Volume, ccSugar, gm 1 1-6 8.77 18.17 62.07 19.76 70 6.3318 2 7-16, BP 41.42 5.9655.51 38.53 84 40.6519 3 17-20, BP 57.21 0 39.51 60.49 49 34.9991 421-25 44.56 0 20.55 79.45 65 34.2789 5 26-30 16.27 0 0 100 60 10.3477 631-35 4.54 0 0 100 45.5 2.0988

EXAMPLE 6

The elution profile shown in FIG. 16 demonstrates of third cycle ofrecycle protocols. The input S-I formats for sample #1 through #5 followsame formats as those demonstrated in previous cycle. The completeloading stage delivers 130 cc feed and spent six minutes for totaltwelve steps or minimal-time intervals.

The formats of input S-I for sequence of recycled streams listed intable 3 of zone 4,5,6, and then eluent water, are identical to thoseillustrated in example 5 covering from samples #6 through #27. Except,the volume for each step is increased from 13.5 cc to 15 cc, i.e., ((4.5cc/5 sec)*3)*1 to ((5 cc/5 sec.)*3)*1 for each step. The consumed volumeof eluent water is 165 cc. The samples #28 through last sample #35follows the same format as illustrated in example 5. The condensed watercollected from condenser is 34 cc to make net of 131 ccwater-consumption. The table 4 shows the group of sample mixturesclassified as zone 1 through zone 6 to cover samples #1 through #35. Themixtures of zone 2 and zone 3 will be considered as by-productabbreviated as BP, namely glucose enriched solution and fructoseenriched solution. The mixture solutions in zone 4,5,6, and 1 will berecycled into the last cycle.

TABLE 4 Zone No. Sample No. D.S. % Oligos % Glucose % Fructose % Volume,cc Sugar, gm 1 1-4 8.56 24.86 60.27 14.87 76 6.7053 2 5-16, BP 43.246.14 52.61 41.25 124 63.1133 3 17-20, BP 55.64 0 29.1 70.9 61.5 42.43114 21-25 35.55 0 11.52 88.48 74 30.0189 5 26-30 10.24 0 0 100 65 6.9016 631-35 2.87 0 0 100 46 1.3335

EXAMPLE 7

The elution profile shown in FIG. 17 demonstrates the last cycle ofrecycle protocols. The input S-I formats for sample #1 through #5 followsame formats as those in second cycle. The complete loading stagedelivers 130 cc feed and spent six minutes for total twelve steps orminimal-time intervals.

The formats of input S-I, the sequence of recycle streams, listed intable 4 of zone 4,5,6, eluent water, and zone 1, follows the same asthose illustrated in last example covering from samples #6 through thelast sample #35. The volume for each step is 15 cc, which is deliveredas ((5 cc/5 sec.)*3)*1 for each step. The consumed volume of eluentwater is 145 cc. The condensed water collected from condenser is 35 ccto make net of 110 cc water-consumption. The table 5 shows the groups ofsample mixtures classified as zone 1 through zone 6 to cover samples #1through #35, considering as various products and by-products (BP).

TABLE 5 Zone Sample No. D.S. % Oligos % Glucose % Fructose % Volume, ccSugar, gm 1 1-4 8.22 29.12 57.79 13.09 74.5 6.3039 2 5-16, BP 43.09 6.7351 42.27 121 61.3310 3 17-20, BP 55.16 0 22.47 77.53 55.3 37.7480 421-25 33.73 0 9.2 90.8 74 28.2774 5 26-30 10.02 0 0 100 68 7.0596 631-35 4.21 0 0 100 50 2.1364

The collected mixtures in zone 4,5, 6, and 1 will be considered as theoutput that can be treated for further usage. The mixture of zone 2 willbe considered as glucose enriched solution and combined with solutionsalready mixed from previous three cycles. While, the mixture from zone 3will be considered as fructose enriched solution and combined withsolution already mixed from the previous three cycles. The table 6summarizes the mixtures of zone 2 and zone 3 for cycle one through cyclefour.

TABLE 6 Zone no. D.S. % Oligos % Glucose % Fructose % Volume, cc Sugar,gms. 2 40.5 6.84 52.89 40.27 383 180.5539 3 53.88 0 35 65 208.8 138.4555

The summation of consumed water for total four cycles is 760 cc withsubtraction of condensed water 132 cc to make the net consumed water is628 cc. The total of feed solution is 520 cc. Thus, the ratio of waterconsumed to feed input is 1.21. The table 7 summarizes the finalizedinformation for cycle one through cycle four of recycle protocols.

TABLE 7 Cycle No. 1 2 3 4 Total steps 45 45 45 45 Input sugar, gm.99.528 135.5675 151.8367 143.8882 Output Sugar, gm. 97.6639 127.9785150.188 141.4285 Recovery % 98.13 94.4 98.91 98.29 Wt. over 90% fructose11.7175 29.5549 48.8862 46.704 Glucose % 9.9 8.9 10.2 9.2 Fructose %90.1 91.1 89.8 90.8 90% fructose recovery 12%  23.1% 32.55% 33.02%

EXAMPLE 8

The FIG. 18 demonstrates the reiterated flow path of liquid streams. Asprevious illustration of differential set-up between two phases and newmass transfer method, each apparatus is the template to finalize theexecution the predetermined elution profile and each separation cycle iscompleted within every spent of minimal time interval. The flow passagesof various liquids and corresponding volume simultaneously deliveredinto each cell of different cycles is derived from the elution profilevia said differential set-up between two phases, of which illustrated inexample 4 through example 7. Four units of preferred apparatus arrangedin series to denote each cycle; having each unit arranged to have saidforty-five steps, or forty-five minimal-time intervals, to cover sixzones. The table 8 summarizes the number of steps in each zone for eachcycle that are the number of cells or group of cells installed in eachzone of each apparatus.

TABLE 8 No. of steps in Cycle 1 Cycle 2 Cycle 3 Cycle 4 Zone 1 16 12 1212 Zone 2 10 14 14 14 Zone 3 4 4 4 4 Zone 4 5 5 5 5 Zone 5 5 5 5 5 Zone6 5 5 5 5

Each cycle needs to spend the said procedures of startup stage forsteady state operation. At steady state operation, each unit isindependent from each other, and yet, the integration from unit onethrough unit four via holding tanks and line connections, exhibits acontinuous separation process including concentration enhancement ofseparated fractions. The zone 1 of each cycle is simultaneously inputwith predetermined feed solution and summation of all liquid volume isequivalent to the throughput requirement. The zone 2, 3, 4, 5, and 6 ofcycle 1 and each corresponding zone 5 in cycle 2,3 and 4, aresimultaneously input with eluent water. The current cycle means cycleone through cycle three and next cycle means next cycle of specificcurrent cycle, individually. The output of each zone 1 in current cycleis simultaneously recycled as input for zone 6 in next cycle. The outputof zone 4,5 and 6 from current cycle are simultaneously recycled asinput into zone 2,3, and 4 of next cycle. The output of zone 2 and zone3 collected from cycle 1 through 4 are mixed as glucose enrichedsolution and fructose enriched solution, respectively.

In the last cycle 4, the output collected from zone 5 and 6 is productof 100% fructose respectively. The output collected from zone 4 is 90%,or plus, fructose solution. The output collected from zone 1 is dilutedglucose solution, which contains the majority of oligosaccharideoriginal in feed solution. Alternatively, the dilute glucose solutioncan be recycled for zone 6 of cycle one to replace water elution forwater conservation. The information of all products obtained under thesespecific running conditions is tabulated in table 5,6 and 7, which arepurposely used for the demonstration of recycle protocols. However, theapplication of the recycle protocols achieves the superiority of betterefficiency in separation of HFCS than the present chromatography processis governed within the general scope of this disclosure either. Thecomparison between the present disclosure and chromatography process isfocused on recovery percentage, resin and eluent water consumption basedon the same feed input rate.

The design summary of current chromatography process for handling 200gallons per minute throughput consumes total 123,420 gallons resin. Thefeed solution is same composition as feed applied in previous examples.There have four columns; each column is 14 feet in diameter and 27.5feet in height, loading with 4125 cubic-ft that is equal to 30,855gallons per column, or, 123,420 gallons total resin consumption. Theprocess requires the input-rate of 350 gallons per minute eluent waterto receive 88% product recovery.

Shown as following, the resin consumption for the separation of HFCS ofpresent disclosure is calculated based same throughput requirement andthe results obtained from cycle 1 through cycle 4 of earlier illustratedexamples. Note that the 0.32 is the ratio of input feed volume to resinbed volume for all four cycles illustrated previously.

Total steps in loading stage is 12 steps, each step is 30 seconds.Except for cycle-one has sixteen steps, but cycle 1 has same forty-fivesteps as other cycles. Actually, each unit in series of four cycles isindependent from each other and communicated via holding tanks andpipelines among individual zones and cycles. Thus, a 200 gal./min.throughput will be evenly divided by four cycles, which are 50gal./min/cycle or 25 gal./30 sec./cycle. Thirty seconds is thepredetermined minimal time interval. The feed volume delivered for eachcell in each cycle is 2.083 gal./step, or 2.083 gal./30 seconds intoeach cell in loading zone. The resin loaded into each cell in theloading zone is (2.083/0.32)*12/step, or, 78.113 gal./cell to cover 12steps for loading zone in each cycle. Thus, the resin inventory is78.113*45, which is equivalent to 3515.09 gallons per cycle. Four cyclesof the recycle protocols will consume 3515.09*4, which is 14,060.36gallons. In comparison to the earlier summary of chromatography process,it requires 123,420 gallons resin stock. The present disclosurerepresented by the running conditions shown in previous examples takesonly 11.4% of such resin stock to handle same capacity requirement.Furthermore, the feed to eluent water ratio shown in example 7 is 1.21.Therefore, the present disclosure will consume 242 gallons per minute ofeluent water base on 200 gallons feed throughput. As shown in table 7,the average recovery percentage from cycle 1 through cycle 4 is 97.41%compare to 88% from chromatography process. Note that the feed solutionis 60% in dry solid base, which is same as chromatography process.

I claim:
 1. A method for separating a plurality of components containedin a first mobile phase comprising a liquid solution of said componentsby contacting a solid phase packing material with the first mobilephase, so that the components are adsorbed by the solid phase material,and subsequently contacting the solid phase with at least a second andthird mobile phase in sequence, each of the second and third mobilephases comprising a liquid eluent of predetermined composition such thatat least two of the components are separately desorbed from the solidphase by the second and third mobile phases, and wherein the solid phaseis contained and in contact with the wall of a cell having an inlet onone side of the solid phase packing material and an outlet on anotherside of the solid phase packing material; by starting with said firstmobile phase material until the solid phase is loaded with thecomponents of the first mobile phase, the method comprising: (a)intermittently delivering predetermined amounts of said first mobilephase material to the inlet of the cell; (b) intermittently supplyingpressurized gas to the cell on the one side of the solid phase packingmaterial following each delivery of said first mobile phase to the inletof the cell in step (a) for increasing the flow rate of the mobile phasethrough the solid phase packing material; (c) maintaining a vacuum onthe other side of the solid phase packing material so as to maintain thesolid phase packing material in a semi-dry state during steps (a) and(b); (d) intermittently collecting a remaining portion of each deliveredamount of said first mobile phase from the outlet of the cell afterpassage through the solid phase packing material; after thepredetermined amounts of said first mobile phase material areintermittently delivered, (e) intermittently delivering predeterminedamounts of said second mobile phase material to the inlet of the cells;(f) intermittently supplying pressurized gas to the cell on the one sideof the solid phase packing material following each delivery of saidsecond mobile phase to the inlet of the cell in step (e) for increasingthe flow rate of the mobile phase through the solid phase packingmaterial; (g) maintaining a vacuum on the other side of the solid phasepacking material so as to maintain the solid phase packing material in asemi-dry state during steps (e) and (f); (h) intermittently collecting aremaining portion of each delivered amount of said second mobile phasefrom the outlet of the cell after passage through the solid phasepacking material; after the predetermined amounts of said second mobilephase material are intermittently delivered, then; (i) intermittentlydelivering predetermined amounts of said third mobile phase material tothe inlet of the cell; (j) intermittently supplying pressurized gas tothe cell on the one side of the solid phase packing material followingseach delivery of said third mobile phase to the inlet of the cell instep (i) for increasing the flow rate of the mobile phase through thesolid phase packing material; (k) maintaining a vacuum on the other sideof the solid phase packing material so as to maintain the solid phasepacking material in a semi-dry state during steps (i) and (j); (l)intermittently collecting a remaining portion of each delivered amountof said third mobile phase from the outlet of the cell after passagethrough the solid phase packing material.
 2. The method of claim 1wherein each intermittent delivery of mobile phase in steps (a), (e),and (i) comprise delivering the respective mobile phase for a first timeperiod, each intermittent supplying pressurized gas in steps (b), (f),and (j) comprise supplying pressurized gas for a second time periodimmediately following the corresponding first time period, and eachintermittent collection of mobile phase in steps (d), (h), and (l)comprise collecting the respective mobile phase for a third time periodimmediately following the corresponding second time period; and whereinthe sum of the first, second, and third time periods defines a minimaltime interval, Δt.
 3. The method of claim 2 wherein the solid phasepacking material is a particulate material retained on a porous meshscreen.
 4. The method of claim 3 wherein the inlet of the cell is abovethe outlet, and wherein steps (a), (e), and (i) comprise intermittentlydelivering the predetermined amounts of the respective mobile phase toslide downward along the wall of the cell to uplift the particulatematerial to form a partially fluidized bed effect for instantaneous andhomogeneous mass transfer contact between said predetermined amount ofthe respective mobile phase and said solid phase packing material,wherein steps (b), (f), and (j) comprise force draining of deliveredrespective mobile phase through said solid phase packing material, andwherein the mass transfer contact is carried out during each sum of thefirst time period and the second time period.
 5. The method of claim 1wherein the solid phase packing material is a particulate ion-exchangeresin.
 6. A method for separating one component from a liquid phase feedsolution containing said one component mixed with at least one othercomponent by sorption and desorption from a permeable adsorbent solidphase packing material, each component having a different iso-pointequilibrium state at which the adsorbed component starts to desorb fromthe solid phase packing material so that desorption of individualcomponents occurs sequentially, wherein the permeable adsorbent solidphase packing material is contained and in contact with wall of at leastone container that has an inlet on one side of the solid phase packingmaterial and an outlet on another side of the solid phase packingmaterial, and wherein at least one particular pressure level is appliedrespectively onto a surrounding space of the inlet and outlet of thecontainer for liquid drainage, the method comprising: providing apredetermined amount of the permeable adsorbent solid phase packingmaterial in at least one container in a semi-dry condition, whereinsemi-dry condition is defined as the packing material having wetsurfaces but with no liquid filling interstices of the material,delivering a preselected amount of said feed solution onto the packingmaterial in multiple intermittent increments so that componentsdissolved in the solution are adsorbed by the packing material,simultaneously draining solvent from each delivered increment of feedsolution from the packing material while maintaining a vacuum at theoutlet of the container so as to maintain the packing material at alltimes in said semi-dry condition, delivering a selected amount of a atleast one eluting liquid having a particular mobile phase compositiononto the packing material in multiple intermittent increments so thatcomponents of the liquid phase feed solution adsorbed by the materialare sequentially desorbed from the packing material into the incrementsof eluting liquid, simultaneously draining each solute-containingincrement of eluting liquid from the packing material while maintaininga vacuum at the outlet of the container so as to maintain the materialat all times in said semi-dry condition, and collecting increments ofdrained eluting liquid containing desorbed amounts of said one componentseparately from increments of drained solvent and drained eluting liquidcontaining desorbed amounts of said at least one other component.
 7. Themethod of claim 6 wherein the predetermined amount of permeable solidphase packing material is an amount to completely adsorb at least saidone component dissolved in the preselected amount of feed solution. 8.The method of claim 7 wherein the predetermined amount of permeablesolid phase packing material is an amount to completely adsorb all ofthe components dissolved in the preselected amount of feed solution. 9.The method of claim 6 wherein the surrounding space of liquid drainedfrom the packing material is maintained at lower pressure than thesurrounding space of liquid delivered onto the packing material, so asto shorten the drainage time of each delivered liquid increment andfacilitate maintaining the packing material in a semi-dry condition. 10.The method of claim 9 wherein the surrounding space of liquid drainedfrom the packing material is maintained under vacuum.
 11. The method ofclaim 6 wherein the pressure of the surrounding space of liquiddelivered onto the packing material is increased after each deliveredincrement of liquid.
 12. The method of claim 6 wherein the surroundingspace of liquid drained from the packing material is maintained undervacuum, and the pressure of the surrounding space of liquid deliveredonto the packing material is increased after each delivered increment ofliquid, so as to shorten the drainage time of each delivered liquidincrement and facilitate maintaining the packing material in a semi-drycondition.
 13. The method of claim 6 wherein the permeable adsorbentsolid phase packing material is a particulate adsorbent resin.
 14. Themethod of claim 13 wherein each increment of feed solution is splashedin an umbrella shape that hits the container wall and slides downward topenetrate and partially uplift the particles of resin suspended inliquid to obtain quick contact and to reduce time for mass transferbetween the delivered liquid and resin.
 15. The method of claim 14wherein each increment of eluting liquid is splashed in an umbrellashape that hits the container wall and slides downward to penetrate andpartially uplift the particles of resin suspended in liquid to obtainquick contact and to reduce time for mass transfer between the deliveredliquid and resin.
 16. The method of claim 6 wherein said at least oneother component in the feed solution comprises a first other componenthaving an iso-point that is before the iso-point of said one component,wherein the iso-point is defined as an equilibrium state that aparticulate mobile phase composition interacts with an adsorbedcomponent, among said one component and at least one other componentthat have been adsorbed by the solid phase packing material, at whichsuch an adsorbed component starts to desorb from the solid phase packingmaterial, so that desorption of an individual component occurssequentially with different predetermined composition of mobile phase;and said at least one eluting liquid comprises a first eluting liquid,having a preset mobile phase composition prior to the iso-point of saidone component that can desorb said first other component, and a secondeluting liquid, having a preset mobile phase composition that justelutes said one component, and a preselected amount of the first elutingliquid is delivered before delivering a preselected amount of the secondeluting liquid.
 17. The method of claim 16 wherein said at least oneother component in the feed solution comprises a second other componenthaving an iso-point that is after the iso-point of said one component,wherein the iso-point is defined as an equilibrium state that aparticulate mobile phase composition interacts with an adsorbedcomponent, among said one component and at least one other componentthat have been adsorbed by the solid phase packing material, at whichsuch an adsorbed component starts to desorb from the solid phase packingmaterial, so that desorption of an individual component occurssequentially with different predetermined composition of mobile phase;and said at least one eluting liquid comprises a third eluting liquid,having a preset mobile phase composition after the iso-point of said onecomponent that can desorb said second other component, and a preselectedamount of the third eluting liquid is delivered after delivering thepreselected amount of the second eluting liquid.
 18. The method of claim17 wherein the preselected amount of the first elution liquidaccomplishes elution of said first other component from the packingmaterial, the preselected amount of the second elution liquidaccomplishes elution of said one component from the packing material,and the preselected amount of the third elution liquid accomplisheselution of said second other component from the packing material. 19.The method of claim 6, wherein the method further comprises: aftercompleting collecting increments of drained eluting liquid containingdesorbed amounts of said one component separately from increments ofdrained solvent and drained eluting liquid containing desorbed amountsof said at least one other component, delivering a preselected amount ofdrained solvent collected from the previously delivered increments offeed solution onto the packing material to wash the material inpreparation for another operation cycle.
 20. The method of claim 6wherein the surrounding space of liquid drained from the packingmaterial and the surrounding space of liquid delivered onto the packingmaterial are maintained at equal pressure and the delivered liquid isdrained by gravity, so as to maintain the packing material in a semi-drycondition.
 21. The method of claim 6 wherein the solid phase packingmaterial is a particulate ion-exchange resin.